LGALS8 Human

Galectin-8 Human Recombinant
Cat. No.
BT11393
Source
Escherichia Coli.
Synonyms
Galectin-8, Gal-8, Po66 carbohydrate-binding protein, Po66-CBP, Prostate carcinoma tumor antigen 1, PCTA-1, LGALS8.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Galectin-8 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 317 amino acids and having a molecular mass of 35.8kDa.
The LGALS8 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Galectins are a family of animal lectins that bind to beta-galactosides. There are at least 14 known members of this family. All galectins share a conserved carbohydrate recognition domain (CRD). They are primarily synthesized in the cytoplasm and lack a typical signal peptide for secretion. However, they can be secreted through unconventional pathways or transported to the nucleus. Galectins play a role in regulating interactions between cells and between cells and the extracellular matrix. Galectin-8, a tandem-repeat-type galectin, comprises two CRDs connected by a linker peptide. Its expression is elevated in lung carcinomas, various prostate cancer types, and other tumor cells. Galectin-8 binds to specific cell surface integrins, thereby influencing integrin-ECM interactions. When immobilized, it promotes cell adhesion by binding and clustering integrin receptors on the cell surface. Conversely, as a soluble ligand, it can inhibit cell adhesion.
Description
Recombinant Human Galectin-8, expressed in E. coli, is a single, non-glycosylated polypeptide chain containing 317 amino acids. It has a molecular weight of 35.8 kDa. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile, white lyophilized powder.
Formulation
LGALS8 was lyophilized from a 1 mg/ml solution in 20 mM PBS at pH 7.4.
Solubility
Reconstitute the lyophilized Galectin-8 in sterile 18 MΩ cm H2O to a concentration of at least 100 µg/ml. The reconstituted solution can be further diluted in other aqueous solutions.
Stability
Lyophilized LGALS8 is stable at room temperature for up to 3 weeks. However, it is recommended to store the lyophilized protein desiccated at -18°C or below. After reconstitution, store Galectin-8 at 4°C for 2-7 days. For long-term storage, freeze the solution at -18°C or below. Avoid repeated freeze-thaw cycles.
Purity
Greater than 95% as determined by: (a) RP-HPLC analysis (b) SDS-PAGE analysis.
Biological Activity
The ED50 of Galectin-8, as measured by its ability to agglutinate human red blood cells, is 0.8-4 µg/ml.
Synonyms
Galectin-8, Gal-8, Po66 carbohydrate-binding protein, Po66-CBP, Prostate carcinoma tumor antigen 1, PCTA-1, LGALS8.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Met-Leu-Ser-Leu.

Product Science Overview

Introduction

Galectin-8, also known as prostate carcinoma tumor antigen 1 (PCTA1), is a member of the galectin family of proteins. Galectins are a group of β-galactoside-binding proteins that play crucial roles in various biological processes, including cell adhesion, migration, proliferation, and apoptosis. Galectin-8 is a tandem-repeat type galectin, meaning it contains two carbohydrate recognition domains (CRDs) connected by a linker peptide .

Structure and Expression

Galectin-8 was initially cloned from a rat liver cDNA library and is composed of 316 amino acids with a molecular weight of approximately 35 kDa . The protein is highly expressed in various tissues, including lung carcinomas, certain forms of prostate carcinomas, and other tumor cells . It is also expressed in the villous and extravillous trophoblast cells of the human placenta, indicating its role in pregnancy establishment .

Biological Functions

Galectin-8 is involved in a wide range of biological functions:

  • Cell Adhesion and Migration: Galectin-8 promotes cell adhesion and migration by interacting with β-galactosides on cell surface glycoproteins and the extracellular matrix . This function is particularly important in processes such as tissue regeneration and cancer metastasis.
  • Proliferation and Apoptosis: Galectin-8 modulates cell proliferation and apoptosis, contributing to its role in cancer progression .
  • Angiogenesis: Galectin-8 plays a key role in angiogenesis, the formation of new blood vessels, which is crucial for tumor growth and wound healing .
  • Trophoblast Invasion: In the context of pregnancy, galectin-8 stimulates the adhesion, migration, and invasion of extravillous trophoblast cells, which are essential for the proper implantation and development of the placenta .
Recombinant Human Galectin-8

Recombinant human galectin-8 is produced using E. coli expression systems. The recombinant protein is typically purified to a high degree of purity (>97%) and is used in various research applications . It is available in both carrier-free and carrier-containing formulations, with the carrier protein (usually bovine serum albumin) enhancing protein stability and shelf-life .

Applications in Research

Recombinant human galectin-8 is widely used in research to study its various biological functions. It is used in cell-based assays to investigate its effects on cell adhesion, migration, proliferation, and apoptosis. Additionally, it is used in studies related to cancer, tissue regeneration, and pregnancy .

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