LGALS8 Human, His
Galectin-8 Human Recombinant, His Tag
Cat. No.
BT11469
Source
Escherichia Coli.
Synonyms
Gal-8, PCTA1, Po66-CBP, Prostate carcinoma tumor antigen 1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
LGALS8 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 337 amino acids (1-317 a.a.) and having a molecular mass of 37.9 kDa. The LGALS8 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
LGALS8, a member of the galectin family, serves as a prostate-specific antigen. Its overexpression is exclusively observed in malignant tumors, making it a valuable supplementary marker for identifying malignancies. LGALS8, similar to the selectin family within C-type lectins, mediates both cell-cell and cell-matrix interactions.
Description
Recombinant human LGALS8, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 337 amino acids (specifically, amino acids 1-317) and exhibits a molecular weight of 37.9 kDa. This LGALS8 protein is engineered with a 20 amino acid His-Tag at its N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution that has been filtered for sterility.
Formulation
The Galectin-8 His tag protein solution is supplied at a concentration of 0.5mg/ml. It is formulated in a buffer containing 20mM Tris-HCl (pH 8), 0.1M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein like HSA or BSA (0.1%). It's important to minimize freeze-thaw cycles to maintain product integrity.
Purity
The purity of the protein is determined using SDS-PAGE analysis and is consistently greater than 90%.
Biological Activity
The biological activity of LGALS8 is assessed by its ability to agglutinate human red blood cells. The ED50 for this agglutination effect is in the range of 5-10 µg/ml. This corresponds to a specific activity of 100-200 IU/mg.
Synonyms
Gal-8, PCTA1, Po66-CBP, Prostate carcinoma tumor antigen 1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MMLSLNNLQN IIYNPVIPFV GTIPDQLDPG TLIVIRGHVP SDADRFQVDL QNGSSMKPRA DVAFHFNPRF
KRAGCIVCNT LINEKWGREE ITYDTPFKRE KSFEIVIMVL KDKFQVAVNG KHTLLYGHRI GPEKIDTLGI YGKVNIHSIG FSFSSDLQST
QASSLELTEI SRENVPKSGT PQLRLPFAAR LNTPMGPGRT VVVKGEVNAN AKSFNVDLLA GKSKDIALHL NPRLNIKAFV RNSFLQESWG
EEERNITSFP FSPGMYFEMI IYCDVREFKV AVNGVHSLEY KHRFKELSSI DTLEINGDIH LLEVRSW.
KRAGCIVCNT LINEKWGREE ITYDTPFKRE KSFEIVIMVL KDKFQVAVNG KHTLLYGHRI GPEKIDTLGI YGKVNIHSIG FSFSSDLQST
QASSLELTEI SRENVPKSGT PQLRLPFAAR LNTPMGPGRT VVVKGEVNAN AKSFNVDLLA GKSKDIALHL NPRLNIKAFV RNSFLQESWG
EEERNITSFP FSPGMYFEMI IYCDVREFKV AVNGVHSLEY KHRFKELSSI DTLEINGDIH LLEVRSW.
Product Science Overview
Introduction
Galectin-8 is a member of the galectin family, which are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. These proteins play crucial roles in various biological processes, including cell-cell adhesion, cell-matrix interactions, growth regulation, apoptosis, and immune responses.
Structure and Expression
The recombinant human Galectin-8 protein, tagged with a His-tag at the N-terminus, is expressed in Escherichia coli and purified using conventional chromatography techniques . The His-tag facilitates the purification process by allowing the protein to bind to nickel ions, making it easier to isolate from other cellular components.
Molecular Characteristics
- Amino Acid Sequence: The recombinant Galectin-8 protein consists of 317 amino acids, with an additional 20 amino acid His-tag at the N-terminus .
- Molecular Weight: The predicted molecular weight of the His-tagged Galectin-8 is approximately 37.9 kDa .
- Purity: The protein is purified to a level greater than 90% as determined by SDS-PAGE .
Biological Functions
Galectin-8 has been implicated in numerous essential functions:
- Development and Differentiation: It plays a role in the development and differentiation of various cell types.
- Cell Adhesion: Galectin-8 mediates cell-cell and cell-matrix adhesion, which is crucial for tissue formation and maintenance.
- Growth Regulation: It is involved in the regulation of cell growth and proliferation.
- Apoptosis: Galectin-8 can induce apoptosis in certain cell types, contributing to the regulation of cell turnover.
- Immune Response: It plays a role in modulating immune responses, including the activation and differentiation of immune cells .
Applications
Recombinant Galectin-8 is used in various research applications, including:
- Cell Biology: Studying cell adhesion, migration, and signaling pathways.
- Cancer Research: Investigating the role of Galectin-8 in tumor progression and metastasis.
- Immunology: Exploring its function in immune cell activation and regulation.
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