Leptin Human, N82K PEG
Greater than 99.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by SDS-PAGE.
Description
Pegylated Leptin N82K Human Recombinant produced in E.Coli is a single non-glycosilated polypeptide chain containing 146 amino acids, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus acids and having a molecular weight of 35.6kDa. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 48 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. Pegylated Leptin N82K Human Recombinant was purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Leptin is a hormone predominantly made by adipose cells and enterocytes in the small intestine that helps to regulate energy balance by inhibiting hunger, which in turn diminishes fat storage in adipocytes. The leptin hormone plays a crucial role in regulating body weight, metabolism, and reproductive functions.
Leptin N82K is a mutant form of the human leptin protein. The mutation involves the substitution of asparagine (N) with lysine (K) at the 82nd position of the amino acid sequence. This mutation can affect the protein’s function and its interaction with the leptin receptor.
Recombinant leptin is produced using recombinant DNA technology, where the leptin gene is inserted into a host organism, such as Escherichia coli (E. coli), to produce the protein. This method allows for the large-scale production of leptin for research and therapeutic purposes.
Pegylation refers to the process of attaching polyethylene glycol (PEG) chains to a protein or peptide. This modification can enhance the stability, solubility, and half-life of the protein in the bloodstream. Pegylated leptin N82K has a PEG molecule attached to its N-terminus, which increases its molecular weight and hydrodynamic volume, resulting in improved pharmacokinetic properties.
- Structure: Pegylated Leptin N82K is a single non-glycosylated polypeptide chain containing 146 amino acids, with an additional alanine (Ala) at the N-terminus and one molecule of PEG 20 kDa at its N-terminus .
- Molecular Weight: The molecular weight of Pegylated Leptin N82K is approximately 35.6 kDa. However, due to its enlarged hydrodynamic volume, it runs on SDS-PAGE as a 48 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein .
- Production: The protein is produced in E. coli and purified using proprietary chromatographic techniques .
- Purity: The purity of Pegylated Leptin N82K is greater than 99.0%, as determined by gel filtration analysis and SDS-PAGE .
- Biological Activity: The biological activity of Pegylated Leptin N82K is less than 0.1%, as determined by inducing proliferation of BAF/3 cells stably transfected with the long form of the human leptin receptor . This reduced activity is due to the drastically reduced affinity toward the leptin receptor.
Pegylated Leptin N82K is primarily used for laboratory research purposes. It is utilized in studies related to energy balance, metabolism, obesity, and leptin receptor interactions. The pegylation of leptin N82K allows researchers to investigate the effects of prolonged leptin activity and its potential therapeutic applications.
- Lyophilized Form: Pegylated Leptin N82K is supplied as a sterile filtered white lyophilized (freeze-dried) powder .
- Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, at a concentration of not less than 100 µg/ml .
- Storage Conditions: The lyophilized protein is stable at room temperature for up to 3 weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, it should be stored at 4°C for 2-7 days and below -18°C for future use . To prevent freeze-thaw cycles, it is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage .
