LDHB Human, His

The human recombinant LDHB with a His tag is a bioactive protein that corresponds to the amino acids 1-334 of the human LDHB . It is expressed in Escherichia coli (E. coli) and purified using conventional chromatography techniques . The His tag, typically located at the N-terminus, facilitates the purification process by allowing the protein to bind to nickel or cobalt ions during affinity chromatography .

LDHB is an oxidoreductase enzyme that plays a pivotal role in cellular respiration and energy production. It is involved in the conversion of pyruvate to lactate, a critical step in anaerobic glycolysis, which allows cells to produce energy under low oxygen conditions . The specific activity of the recombinant LDHB is greater than 300 units/mg, where one unit converts 1.0 µmole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C .

Recombinant LDHB is widely used in research to study metabolic pathways, enzyme kinetics, and cellular respiration. It serves as a powerful marker for germ cell tumors and is utilized in various biochemical assays . The His tag aids in the easy purification and detection of the protein, making it a valuable tool in laboratory settings .

For optimal stability, the recombinant LDHB should be stored at 4°C for short-term use and at -20°C for long-term storage. It is recommended to avoid freeze-thaw cycles to maintain the protein’s integrity . The protein is typically stored in a buffer containing 20 mM Tris-HCl (pH 8.0), 10% glycerol, and 1 mM DTT .