LDHB Antibody
Description
Product Specs
Product Science Overview
Lactate Dehydrogenase B (LDHB) is an enzyme that plays a crucial role in the metabolic pathway of glycolysis. It is one of the subunits of the lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with the concomitant interconversion of NADH and NAD+. This process is essential for cellular respiration and energy production, particularly under anaerobic conditions.
LDHB is a protein-coding gene that encodes the B subunit of the lactate dehydrogenase enzyme. The enzyme itself is a tetramer composed of different combinations of two subunits: LDHA (muscle type) and LDHB (heart type). The combination of these subunits determines the enzyme’s kinetic properties and tissue-specific expression. LDHB is predominantly found in the heart, brain, and red blood cells, where it facilitates the conversion of lactate to pyruvate, a critical step in the Cori cycle and gluconeogenesis .
The LDHB gene is located on chromosome 12 in humans and has several alternatively spliced transcript variants. These variants encode different isoforms of the protein, which may have distinct cellular localizations and functions. Mutations in the LDHB gene can lead to lactate dehydrogenase B deficiency, a rare metabolic disorder characterized by myopathy and exercise intolerance .
LDHB is a significant biomarker in various clinical conditions. Elevated levels of LDHB are often associated with tissue damage, hemolysis, and certain types of cancer. For instance, high LDHB expression is observed in renal carcinoma and other malignancies, making it a potential target for cancer diagnostics and therapeutics .
Mouse anti-human LDHB antibodies are monoclonal antibodies produced by immunizing mice with human LDHB protein. These antibodies are highly specific and are used in various research and diagnostic applications, including Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays (ELISA). They are valuable tools for detecting and quantifying LDHB in biological samples, studying its expression patterns, and understanding its role in different physiological and pathological conditions .
Mouse anti-human LDHB antibodies have been instrumental in advancing our understanding of LDHB’s function and regulation. They have been used to investigate the enzyme’s role in metabolic pathways, its involvement in disease mechanisms, and its potential as a therapeutic target. These antibodies also facilitate the study of LDHB’s interaction with other proteins and its regulation by various cellular signals .
