IRF3 Antibody
Description
Product Specs
Product Science Overview
Interferon Regulatory Factor-3 (IRF3) is a critical transcription factor involved in the innate immune response, particularly in the induction of antiviral immunity. It plays a pivotal role in the regulation of type I interferon (IFN) genes and other interferon-stimulated genes (ISGs). The mouse anti-human IRF3 antibody is a monoclonal antibody used in various research applications to study the expression and function of IRF3 in human cells.
IRF3 is a member of the interferon regulatory factor family, which consists of several transcription factors involved in the regulation of the immune response. IRF3 is constitutively expressed in most cells and remains inactive in the cytoplasm of uninfected cells. Upon viral infection or other stimuli, IRF3 undergoes phosphorylation, leading to its dimerization and translocation to the nucleus. In the nucleus, IRF3 binds to specific DNA sequences in the promoters of target genes, initiating the transcription of type I IFNs and ISGs .
IRF3 is essential for the induction of type I IFNs, which are crucial for the antiviral response. Type I IFNs, including IFN-α and IFN-β, are cytokines that play a key role in the defense against viral infections. They activate the expression of ISGs, which inhibit various stages of the viral life cycle. The activation of IRF3 is triggered by the recognition of viral nucleic acids by pattern recognition receptors (PRRs) such as Toll-like receptors (TLRs) and RIG-I-like receptors (RLRs) .
The mouse anti-human IRF3 antibody is a monoclonal antibody that specifically recognizes human IRF3. It is widely used in research to study the expression, localization, and function of IRF3 in human cells. This antibody is suitable for various applications, including Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry .
- Western Blotting: The mouse anti-human IRF3 antibody is used to detect IRF3 protein levels in cell lysates. This technique helps researchers study the regulation of IRF3 expression and its activation in response to viral infections or other stimuli.
- Immunohistochemistry: This antibody is used to visualize the localization of IRF3 in tissue sections. Immunohistochemical analysis has shown that IRF3 expression is highly organ- and cell-type specific, with high expression in certain epithelial cells and low expression in brain parenchyma .
- Immunofluorescence: The antibody is used to study the subcellular localization of IRF3 in cultured cells. Immunofluorescence staining can reveal the translocation of IRF3 from the cytoplasm to the nucleus upon activation.
- Flow Cytometry: The mouse anti-human IRF3 antibody can be used in flow cytometry to analyze the expression of IRF3 in different cell populations. This application is useful for studying the regulation of IRF3 in various immune cell types .
