IFN A WB Antibody

Interferon-alpha Western Blot, Mouse Anti-Human
Cat. No.
BT5915
Source
Synonyms
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Product Specs

Introduction
IFN-alpha, produced by macrophages, exhibits antiviral properties and stimulates the synthesis of protein kinase and oligoadenylate synthetase.
Formulation
Supplied as a 1 mg/ml solution in PBS following reconstitution.
Shipping Conditions
The antibody is shipped in lyophilized form at room temperature.
Storage Procedures
For long-term storage, keep the lyophilized antibody at 4°C in a dry environment. After reconstitution, aliquot and store at -20°C if not intended for use within one month.
Solubility
Reconstitute the antibody with sterile water. Gently mix the solution, ensuring the vial sides are washed, and allow it to stand for 30-60 seconds before use.
Applications
Suitable applications include Direct ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. This antibody does not neutralize its target.
Note
This antibody can be paired with THE BioTek's Mouse Anti-Human IFN-A Neutralizing antibody in Capture ELISA assays.
Titer
A 1:10,000 dilution in a direct ELISA will yield an optical density (OD) of 0.5 when using alkaline phosphatase-conjugated rabbit anti-mouse Ig (Jackson Laboratories).
Purification Method
Ion exchange.
Type
Mouse Anti Human Monoclonal.
Clone
NYRhIFN-A(WB).
Immunogen
r.Human IFN-a.
Ig Subclass
Mouse IgG1.

Product Science Overview

Introduction

Interferon-alpha (IFN-α) is a type of cytokine that plays a crucial role in the immune response against viral infections and certain cancers. It is part of the larger interferon family, which includes interferon-beta (IFN-β) and interferon-gamma (IFN-γ). IFN-α is produced by leukocytes and has multiple subtypes, each encoded by different genes. The detection and analysis of IFN-α are essential in various research and clinical settings, particularly in understanding immune responses and developing therapeutic strategies.

Western Blot Technique

Western blotting is a widely used analytical technique in molecular biology and biochemistry to detect specific proteins in a sample. The process involves the separation of proteins by gel electrophoresis, transfer to a membrane, and detection using specific antibodies. The technique is highly sensitive and allows for the quantification and characterization of proteins.

Mouse Anti-Human IFN-α Antibodies

Mouse anti-human IFN-α antibodies are monoclonal antibodies produced by immunizing mice with human IFN-α proteins. These antibodies are highly specific and bind to IFN-α, allowing for its detection in various assays, including Western blotting. The use of mouse monoclonal antibodies provides consistency and specificity in experimental results.

Applications in Research
  1. Viral Infections: IFN-α is a key player in the antiviral response. Researchers use Western blotting with mouse anti-human IFN-α antibodies to study the expression and regulation of IFN-α in infected cells.
  2. Cancer Research: IFN-α has therapeutic applications in certain cancers. Western blotting helps in understanding the mechanisms of IFN-α action and its effects on cancer cells.
  3. Immunology: The role of IFN-α in modulating the immune response is critical. Researchers use Western blotting to investigate how IFN-α influences immune cell signaling and function.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
© Copyright 2024 Thebiotek. All Rights Reserved.