IFN a 2a Human, Plant
Interferon Alpha 2a Human Recombinant, Tobacco
Cat. No.
BT25991
Source
Nicotiana Sp.Plant.
Synonyms
Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-a 2a.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 99.0% as determined by both:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Interferon Alpha Human 2a Recombinant produced in Plant is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19 kDa.
The Interferon-a 2a contains affinity 6xHis tag on C-terminus.
The IFN-A 2a is purified by proprietary chromatographic techniques.
The Interferon-a 2a contains affinity 6xHis tag on C-terminus.
The IFN-A 2a is purified by proprietary chromatographic techniques.
Product Specs
Introduction
IFN-alpha, produced by macrophages, exhibits antiviral properties by stimulating the production of protein kinase and oligoadenylate synthetase.
Description
Recombinant Human Interferon Alpha 2a, produced in plants, is a single, glycosylated polypeptide chain with a molecular weight of 19 kDa, comprising 165 amino acids. It features a 6xHis affinity tag at the C-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
White, sterile-filtered lyophilized powder.
Formulation
Lyophilized with 50% SDS.
Solubility
Reconstitute the lyophilized Interferon-alpha 2a in sterile 18 megaohm-cm H2O to a concentration of at least 100 micrograms per milliliter. This solution can be further diluted with other aqueous solutions.
Stability
Lyophilized Interferon alpha 2a remains stable at room temperature for up to 3 weeks but should be stored desiccated below -18 degrees Celsius. After reconstitution, store IFN-alpha 2a at 4 degrees Celsius for 2-7 days. For long-term storage, freeze at -18 degrees Celsius after adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 99.0% as determined by both reverse-phase high-performance liquid chromatography (RP-HPLC) and SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
Biological Activity
Specific activity determined using a quantitative gene report bioassay with human Type I interferon-sensitive cells. Plant-derived IFN-alpha 2a exhibited an activity of 6.1 x 10^8 Units/mg, compared to bacterially derived recombinant IFN-a 2a under the same assay conditions, based on a viral resistance assay using bovine kidney MDBK cells.
Synonyms
Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-a 2a.
Source
Nicotiana Sp.Plant.
Amino Acid Sequence
The sequence of the first twelve N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln-Thr-His-Ser-Leu-Gly-Ser-Arg.
Product Science Overview
Introduction
Interferon Alpha 2a (IFN-α2a) is a type of cytokine, a protein that plays a crucial role in the immune response. It is part of the type I interferon family and is known for its antiviral, antiproliferative, and immunomodulatory properties . The recombinant form of Interferon Alpha 2a is produced using various expression systems, including tobacco plants, which is a novel and innovative approach.
Production Process
The production of recombinant Interferon Alpha 2a involves inserting the human interferon gene into a suitable expression system. In this case, the tobacco plant (Nicotiana species) is used as the host. The process includes:
- Gene Insertion: The human interferon gene is inserted into the tobacco plant’s genome using genetic engineering techniques.
- Cultivation: The genetically modified tobacco plants are cultivated under controlled conditions.
- Protein Extraction: The interferon protein is extracted from the plant tissues.
- Purification: The extracted protein undergoes several purification steps to ensure high purity and activity .
This plant-based production system offers several advantages, including lower production costs and the ability to scale up production easily.
Uses
Interferon Alpha 2a is used to treat various medical conditions, including:
- Chronic Hepatitis B and C: It helps in reducing the viral load and improving liver function .
- Certain Cancers: It is used in the treatment of cancers such as leukemia, melanoma, and AIDS-related Kaposi’s sarcoma .
- Other Viral Infections: It is also used to treat other viral infections and conditions like condylomata acuminata .
Mechanism of Action
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