IFNA1A Human
IFN-alpha 1a, IFN-a 1a, IFN alpha 1a.
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
IFN-alpha 1a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 167 amino acids and having a molecular mass of 19.5kDa. The IFNA1A ( V115A ) is purified by proprietary chromatographic techniques.
Product Specs
Recombinant Human IFN-alpha 1a, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 167 amino acids. It has a molecular mass of 19.5 kDa. This IFNA1A (V115A) variant is purified using proprietary chromatographic techniques.
The IFN-a 1a protein was lyophilized from a 0.2µm filtered concentrated solution in PBS (pH 7.4) containing 3% Mannitol, 5% Trehalose, and 0.05% Tween-80.
Lyophilized IFNA1A, while stable at room temperature for 3 weeks, should be stored desiccated at a temperature below -18°C. After reconstitution, IFN-alpha 1a should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to store below -18°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity is greater than 97.0% as determined by:
(a) Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
IFN-alpha 1a, IFN-a 1a, IFN alpha 1a.
MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFP QEEFDGNQFQ KAPAISVLHE LIQQIFNLFT TKDSSAAWDE DLLDKFCTEL YQQLNDLEAC VMQEERVGET PLMNADSILA VKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQ ERLRRKE.
Product Science Overview
Interferon-alpha 1a (IFN-α1a) is a type of recombinant protein that belongs to the family of Type I interferons. Interferons are cytokines with potent antiviral, antiproliferative, and immunomodulatory properties . IFN-α1a is produced using recombinant DNA technology, which involves inserting the human gene encoding IFN-α1a into a host cell, such as E. coli or human embryonic kidney cells (HEK293), to produce the protein .
IFN-α1a exhibits several biological properties:
- Antiviral Activity: It inhibits viral replication within host cells by inducing the expression of antiviral proteins .
- Antiproliferative Effects: It can inhibit the proliferation of various cell types, including tumor cells .
- Immunomodulatory Functions: It enhances the immune response by increasing the activity of natural killer (NK) cells and macrophages, and by promoting the differentiation of T cells .
The mode of action of IFN-α1a involves binding to specific cell surface receptors known as interferon-alpha receptors (IFNAR1 and IFNAR2). This binding triggers a signaling cascade through the JAK-STAT pathway, leading to the transcription of interferon-stimulated genes (ISGs). These genes encode proteins that mediate the antiviral, antiproliferative, and immunomodulatory effects of IFN-α1a .
Recombinant IFN-α1a is typically produced in host cells such as E. coli or HEK293 cells. The protein is then purified using chromatographic techniques to achieve high purity levels, often exceeding 95% . The final product is lyophilized and can be reconstituted in phosphate-buffered saline (PBS) for use in various applications .
