ING2 Human

Inhibitor of Growth Family, Member 2 Human Recombinant
Cat. No.
BT24446
Source
Escherichia Coli.
Synonyms
Inhibitor Of Growth Family Member 2, ING1L, Inhibitor Of Growth 1-Like Protein, P33ING2, ING1Lp, P32, Inhibitor Of Growth Family Member 1-Like, Inhibitor Of Growth Protein 2, ING2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ING2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 303 amino acids (1-280 a.a) and having a molecular mass of 35.2kDa.
ING2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
As a member of the inhibitor of growth (ING) family, Inhibitor of Growth Family, Member 2 (ING2) interacts with and regulates the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes. These complexes play crucial roles in DNA repair and apoptosis. ING2 is believed to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, potentially by enhancing the acetylation of p53/TP53. It is a component of an mSin3A-like corepressor complex, which is likely involved in the deacetylation of nucleosomal histones. The activity of ING2 is modulated by its binding to phosphoinositides (PtdInsPs).
Description
ING2 Human Recombinant, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 303 amino acids (specifically, amino acids 1-280). It possesses a molecular mass of 35.2 kDa. A 23 amino acid His-tag is fused to the N-terminus of ING2, and purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The ING2 protein solution (1 mg/ml) contains 20mM Tris-HCl buffer with a pH of 8.0, 0.4M UREA, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep the vial at 4°C. For longer periods, store frozen at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for long-term storage. It is important to avoid repeated freeze-thaw cycles.
Purity
SDS-PAGE analysis confirms a purity greater than 85.0%.
Synonyms
Inhibitor Of Growth Family Member 2, ING1L, Inhibitor Of Growth 1-Like Protein, P33ING2, ING1Lp, P32, Inhibitor Of Growth Family Member 1-Like, Inhibitor Of Growth Protein 2, ING2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMLGQQQQ QLYSSAALLT GERSRLLTCY VQDYLECVES LPHDMQRNVS VLRELDNKYQ ETLKEIDDVY EKYKKEDDLN QKKRLQQLLQ RALINSQELG DEKIQIVTQM LELVENRARQ MELHSQCFQD PAESERASDK AKMDSSQPER SSRRPRRQRT SESRDLCHMA NGIEDCDDQP PKEKKSKSAK KKKRSKAKQE REASPVEFAI DPNEPTYCLC NQVSYGEMIG CDNEQCPIEW FHFSCVSLTY KPKGKWYCPK CRGDNEKTMD KSTEKTKKDR RSR.

Product Science Overview

Introduction

The Inhibitor of Growth (ING) family comprises tumor suppressor genes that play crucial roles in regulating cell proliferation, apoptosis, and cellular senescence. The family includes five members: ING1, ING2, ING3, ING4, and ING5. These proteins are highly conserved across species and are involved in various cellular processes through their interactions with chromatin and other proteins.

ING2 Gene and Protein

The ING2 gene encodes the Inhibitor of Growth Family, Member 2 protein, also known as p33ING2 . This protein is involved in several critical cellular functions, including DNA repair, apoptosis, and cell cycle regulation. ING2 is known to interact with histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes, modulating their activity and influencing chromatin structure and gene expression .

Functional Domains

ING2 contains several functional domains that enable its interaction with chromatin and other proteins:

  • Plant Homeodomain (PHD) Finger: This domain allows ING2 to bind to methylated histones, particularly histone H3 trimethylated at lysine 4 (H3K4me3), which is associated with active transcription.
  • Leucine Zipper-like Domain: This domain facilitates protein-protein interactions, including those with p53, a key regulator of the cell cycle and apoptosis .
Biological Functions

ING2 plays a pivotal role in various biological processes:

  • DNA Repair: ING2 is involved in the DNA damage response, enhancing the acetylation of p53 and promoting its activation. This leads to the transcription of genes involved in DNA repair and apoptosis .
  • Apoptosis: By modulating p53 activity, ING2 can induce apoptosis in response to cellular stress or DNA damage .
  • Cell Cycle Regulation: ING2 helps regulate the cell cycle by influencing the expression of genes involved in cell proliferation and growth arrest .
Role in Cancer

As a tumor suppressor, ING2 is often downregulated or mutated in various cancers. Its loss of function can lead to uncontrolled cell proliferation and tumor development. Research has shown that restoring ING2 function in cancer cells can inhibit their growth and induce apoptosis, highlighting its potential as a therapeutic target .

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