IMPAD1 Mouse Bioactive
Inositol Monophosphatase Domain Containing 1 Mouse Recombinant Bioactive
Cat. No.
BT24148
Source
Sf9,
Baculovirus cells.
Synonyms
Inositol monophosphatase 3, IMP 3, IMPase 3, Golgi 3-prime
phosphoadenosine 5-prime phosphate 3-prime phosphatase, Golgi-resident PAP
phosphatase, gPAPP, Inositol monophosphatase domain-containing protein 1, Inositol-1(or
4)-monophosphatase 3, Myo-inositol monophosphatase A3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
IMPAD1 Mouse Recombinant produced in Sf9 Baculovirus
cells is a single, glycosylated polypeptide chain containing 332 amino acids (34-356a.a.)
and having a molecular mass of 36.2kDa. (Molecular size on SDS-PAGE will appear
at approximately 28-40kDa).
IMPAD1 is expressed with a
6 amino acid His tag at C-Terminus and purified by proprietary chromatographic
techniques.
Product Specs
Introduction
Inositol monophosphatase 3 (IMPAD1), a member of the inositol monophosphatase family, is localized in the Golgi apparatus. It functions as an enzyme that catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP). Mutations in the IMPAD1 gene are associated with GRAPP type chondrodysplasia, characterized by joint dislocations. Additionally, a pseudogene of IMPAD1 is found on the long arm of chromosome 1.
Description
IMPAD1 Mouse Recombinant, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain containing 332 amino acids (residues 34-356) and has a molecular mass of 36.2 kDa. On SDS-PAGE, the apparent molecular size will be approximately 28-40 kDa. This recombinant protein is expressed with a 6 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The IMPAD1 protein solution is provided at a concentration of 0.5 mg/ml in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For longer periods, it is recommended to store the product frozen at -20°C. To ensure long-term stability, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Multiple freeze-thaw cycles should be avoided.
Purity
The purity of this product is greater than 95.0% as determined by SDS-PAGE analysis.
Biological Activity
The specific activity, a measure of the enzyme's ability to hydrolyze Adenosine 3, 5-diphosphate, is greater than 5,000 pmol/min/µg. This activity is determined at pH 7.5 and 25°C.
Synonyms
Inositol monophosphatase 3, IMP 3, IMPase 3, Golgi 3-prime
phosphoadenosine 5-prime phosphate 3-prime phosphatase, Golgi-resident PAP
phosphatase, gPAPP, Inositol monophosphatase domain-containing protein 1, Inositol-1(or
4)-monophosphatase 3, Myo-inositol monophosphatase A3.
Source
Sf9,
Baculovirus cells.
Amino Acid Sequence
ADPGRFSLFG LGSEPAAGEA
EVASDGGTVD LREMLAVAVL AAERGGDEVR RVRESNVLHE KSKGKTREGA DDKMTSGDVL SNRKMFYLLK TAFPNVQINT
EEHVDASDKE VIVWNRKIPE DILKEIAAPK EVPAESVTVW IDPLDATQEY TEDLRKYVTT MVCVAVNGKP
VLGVIHKPFS
EYTAWAMVDG GSNVKARSSY
NEKTPKIIVS RSHAGMVKQV ALQTFGNQTS IIPAGGAGYK VLALLDVPDM TQEKADLYIH VTYIKKWDIC AGNAILKALG
GHMTTLNGEE ISYTGSDGIE GGLLASIRMN HQALVRKLPD LEKSGHHHHH HH.
Product Science Overview
Structure and Expression
Biological Function
IMPAD1 catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP) . This reaction is essential for maintaining the balance of inositol phosphates within the cell, which are critical for various cellular functions, including signal transduction and membrane biogenesis.
Tissue Distribution
Recombinant Bioactive Form
Research Applications
IMPAD1 is studied for its role in neurodevelopmental disorders and neurodegenerative diseases. Its involvement in inositol phosphate metabolism makes it a potential target for therapeutic interventions aimed at modulating cellular signaling pathways.
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