IMPAD1 Human, Active
Escherichia Coli.
Inositol monophosphatase 3, IMP 3, IMPase 3, EC 3.1.3.25, EC 3.1.3.7, Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime phosphatase, Golgi-resident PAP phosphatase, gPAPP, Inositol monophosphatase domain-containing protein 1, Inositol-1(or 4)-monophosphatase 3, Myo-inositol monophosphatase A3, IMPAD1, IMPA3, GPAPP, IMP-3
Greater than 90.0% as determined by SDS-PAGE.
Description
IMPAD1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 349 amino acids (34-359 a.a.) and having a molecular mass of 37.6kDa.
IMPAD1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Inositol monophosphatase 3, IMP 3, IMPase 3, EC 3.1.3.25, EC 3.1.3.7, Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime phosphatase, Golgi-resident PAP phosphatase, gPAPP, Inositol monophosphatase domain-containing protein 1, Inositol-1(or 4)-monophosphatase 3, Myo-inositol monophosphatase A3, IMPAD1, IMPA3, GPAPP, IMP-3
Escherichia Coli.
MGSSHHHHHH SSGLVPRGSH MGSGRFSLFG LGGEPGGGAA GPAAAADGGT VDLREMLAVS VLAAVRGGDE VRRVRESNVL HEKSKGKTRE GAEDKMTSGD VLSNRKMFYL LKTAFPSVQI NTEEHVDAAD QEVILWDHKI PEDILKEVTT PKEVPAESVT VWIDPLDATQ EYTEDLRKYV TTMVCVAVNG KPMLGVIHKP FSEYTAWAMV DGGSNVKARS SYNEKTPRIV VSRSHSGMVK QVALQTFGNQ TTIIPAGGAG YKVLALLDVP DKSQEKADLY IHVTYIKKWD ICAGNAILKA LGGHMTTLSG EEISYTGSDG IEGGLLASIR MNHQALVRKL PDLEKTGHK
Product Science Overview
IMPAD1 is involved in the hydrolysis of inositol monophosphate to produce free inositol, which is a key component in the synthesis of phosphatidylinositol and its derivatives. These molecules are essential for various cellular processes, including signal transduction, membrane trafficking, and cytoskeletal organization.
Recombinant Human Inositol Monophosphatase Domain Containing 1 (BioActive) is produced using recombinant DNA technology. This involves inserting the human IMPAD1 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. The recombinant protein is then purified and tested for bioactivity to ensure it retains its functional properties.
The bioactive recombinant IMPAD1 protein is used in various research applications, including:
- Biochemical studies: Understanding the enzyme’s role in cellular processes and its regulation.
- Drug discovery: Screening for potential inhibitors or activators of IMPAD1, which could be therapeutic targets for diseases related to phosphatidylinositol signaling.
- Structural biology: Investigating the three-dimensional structure of IMPAD1 to gain insights into its mechanism of action.
Mutations in the IMPAD1 gene have been associated with certain medical conditions, such as chondrodysplasia with joint dislocations, GPAPP type. This highlights the importance of IMPAD1 in normal skeletal development and its potential as a target for therapeutic intervention.
