IL 1RA Rat, His
Description
IL 1RA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
MGSSHHHHHH SSGLVPRGSH MGSHPAGKRP CKMQAFRIWD TNQKTFYLRN NQLIAGYLQG PNTKLEEKID MVPIDFRNVF LGIHGGKLCL SCVKSGDDTK LQLEEVNITD LNKNKEEDKR FTFIRSETGP TTSFESLACP GWFLCTTLEA DHPVSLTNTP KEPCTVTKFY FQEDQ.
Product Science Overview
Interleukin-1 Receptor Antagonist (IL-1Ra) is a naturally occurring cytokine that plays a crucial role in regulating immune and inflammatory responses. It is a member of the interleukin 1 cytokine family and functions by inhibiting the activities of interleukin 1, alpha (IL1A), and interleukin 1, beta (IL1B) . The recombinant form of IL-1Ra, particularly the rat version with a His tag, is widely used in research to study its biological functions and potential therapeutic applications.
The recombinant rat IL-1Ra protein is typically expressed in HEK 293 cells, a human embryonic kidney cell line, which ensures high levels of protein expression and proper post-translational modifications . The His tag, a sequence of histidine residues, is added to the C-terminus of the protein to facilitate purification and detection. This tag allows for easy isolation of the protein using nickel affinity chromatography, a common technique in protein purification.
IL-1Ra functions as a competitive inhibitor of the interleukin-1 receptor (IL1R1). By binding to IL1R1, it prevents the association of the receptor with its coreceptor IL1RAP, thereby inhibiting the signaling pathways activated by IL1A and IL1B . This inhibition is crucial in modulating immune and inflammatory responses, particularly during the acute phase of infection and inflammation .
Recombinant rat IL-1Ra is used extensively in research to study its role in various biological processes and diseases. It is employed in assays such as SDS-PAGE and sELISA to investigate its interactions and functions . Additionally, IL-1Ra has been studied for its potential therapeutic applications in conditions characterized by excessive inflammation, such as rheumatoid arthritis and other autoimmune diseases .
The IL1RN gene, which encodes IL-1Ra, has been associated with several diseases. Polymorphisms in this gene are linked to an increased risk of osteoporotic fractures and gastric cancer . Understanding the function and regulation of IL-1Ra can provide insights into the development of new therapeutic strategies for these conditions.
