IL 1RA Mouse, His

Interleukin-1 Receptor Antagonist Mouse Recombinant, His Tag
Cat. No.
BT1256
Source
E.coli.
Synonyms
IRAP, IL1F3, IL1RA, IL-1ra3, ICIL-1RA, IL1RN, IL1 inhibitor, IL-1ra, F630041P17Rik.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IL-1RA mouse Recombinant produced E. coli is a single polypeptide chain containing 177 amino acids (27-178) and having a molecular mass of 20kDa.
IL-1RA is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin-1 receptor antagonist (IL-1RA) is a protein that plays a crucial role in regulating inflammatory responses. It belongs to the interleukin 1 cytokine family and acts by inhibiting the activity of interleukin 1 alpha (IL1A) and interleukin 1 beta (IL1B). By blocking these pro-inflammatory cytokines, IL-1RA helps to modulate a wide range of immune and inflammatory responses. The gene encoding IL-1RA, along with five other related cytokine genes, forms a cluster on chromosome 2. Genetic variations in this gene have been associated with an increased risk of conditions like osteoporosis and gastric cancer. Notably, four different isoforms of IL-1RA can be produced from the same gene through a process called alternative splicing.
Description
Recombinant IL-1RA from mice, produced in E. coli, is a single polypeptide chain comprising 177 amino acids (residues 27-178) with a molecular weight of 20 kDa. A 25 amino acid His-tag is fused to the N-terminus of IL-1RA to facilitate purification by proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The IL-1RA solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.1M NaCl, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the IL-1RA solution can be stored at 4°C. For longer storage, it is recommended to freeze the solution at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein like HSA or BSA to a final concentration of 0.1% is advisable. It is important to avoid repeated freeze-thaw cycles to prevent protein degradation.
Purity
The purity of the IL-1RA protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
IRAP, IL1F3, IL1RA, IL-1ra3, ICIL-1RA, IL1RN, IL1 inhibitor, IL-1ra, F630041P17Rik.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMRPSGK RPCKMQAFRI WDTNQKTFYL RNNQLIAGYL QGPNIKLEEK IDMVPIDLHS VFLGIHGGKL CLSCAKSGDD IKLQLEEVNI TDLSKNKEED KRFTFIRSEK GPTTSFESAA CPGWFLCTTL EADRPVSLTN TPEEPLIVTK FYFQEDQ.

Product Science Overview

Introduction

Interleukin-1 Receptor Antagonist (IL-1Ra) is a naturally occurring protein that plays a crucial role in modulating the immune response by inhibiting the activities of interleukin-1 (IL-1) cytokines. The recombinant form of IL-1Ra, particularly the mouse recombinant version with a His tag, is widely used in research to study its effects and potential therapeutic applications.

Structure and Production

The mouse recombinant IL-1Ra is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 177 amino acids. It has a molecular mass of approximately 20 kDa. The protein is fused to a 25 amino acid His tag at the N-terminus, which facilitates its purification through chromatographic techniques .

Function and Mechanism

IL-1Ra functions as an anti-inflammatory antagonist of the interleukin-1 family of proinflammatory cytokines, such as IL-1β and IL-1α. By binding to the IL-1 receptors without eliciting a signal, IL-1Ra effectively blocks the activities of IL-1, thereby protecting the body from immune dysregulation and uncontrolled systemic inflammation triggered by various stimulatory agents, including pathogens .

Applications in Research

Recombinant IL-1Ra has been extensively studied for its potential therapeutic applications. For instance, it has been shown to provide cardioprotection during myocardial ischemia-reperfusion injury in mice. Pretreatment with recombinant human IL-1Ra (rhIL-1Ra) has demonstrated marked cardioprotective effects by preserving left ventricular ejection fraction and reducing infarct size .

Clinical Relevance

The IL1RN gene, which encodes IL-1Ra, is associated with various diseases, including chronic recurrent multifocal osteomyelitis and microvascular complications of diabetes. Polymorphisms in this gene have been linked to an increased risk of osteoporotic fractures and gastric cancer . Understanding the function and therapeutic potential of IL-1Ra is crucial for developing treatments for these conditions.

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