IL 1RA Horse

Interleukin-1 Receptor Antagonist Horse Recombinant
Cat. No.
BT850
Source
Escherichia Coli.
Synonyms
Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor, IL1RN, IL1RA.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant Horse Interleukin-1 Receptor Antagonist produced in E.coli cells is a single, non-glycosylated, polypeptide chain containing 152 amino acids and having a molecular mass of 17.4kDa.
The IL-1RA is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin-1 receptor antagonist (IL-1RA) is a protein belonging to the interleukin 1 cytokine family. It acts by blocking the activity of interleukin-1 alpha (IL1A) and interleukin-1 beta (IL1B), thereby regulating various immune and inflammatory responses associated with interleukin-1. The gene encoding IL-1RA, along with five other structurally similar cytokine genes, forms a cluster spanning roughly 400 kb on chromosome 2. Polymorphisms in this gene have been linked to an elevated risk of osteoporosis-related fractures and gastric cancer. Four alternatively spliced transcript variants, which produce distinct isoforms of IL-1RA, have been identified.
Description
Recombinant Horse Interleukin-1 Receptor Antagonist is a single, non-glycosylated polypeptide chain comprising 152 amino acids, resulting in a molecular weight of 17.4 kDa. It is produced in E.coli cells. The purification process involves the use of proprietary chromatographic techniques to ensure high purity.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The IL-1RA undergoes lyophilization from a 0.2 µm filtered solution concentrated in phosphate-buffered saline (PBS) at pH 7.4.
Solubility
To reconstitute the lyophilized IL-1RA, it is recommended to dissolve it in sterile water to a minimum concentration of 100 µg/ml. This solution can be further diluted using other aqueous solutions as needed.
Stability
Lyophilized IL-1RA demonstrates stability at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store it in a desiccated state below -18°C. Once reconstituted, IL-1RA should be stored at 4°C for a period of 2-7 days. For long-term storage, it is advisable to add a carrier protein such as 0.1% HSA or BSA. It is important to avoid repeated freeze-thaw cycles to maintain stability.
Purity
The purity of the recombinant Horse Interleukin-1 Receptor Antagonist is greater than 95.0%, as determined by two methods: Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Biological Activity
The biological activity of the recombinant Horse Interleukin-1 Receptor Antagonist is determined by its ability to inhibit IL-1α-dependent proliferation of murine D10.G4.1 helper T cells. The ED50, representing the concentration at which 50% inhibition is observed, is less than 3.0 µg/ml. This corresponds to a specific activity greater than 333 IU/mg in the presence of 50 pg/ml recombinant human IL-1α.
Synonyms
Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor, IL1RN, IL1RA.
Source
Escherichia Coli.
Amino Acid Sequence
HPLGKRPCKM QAFRIWDVNQ KTFYMRNNQL VAGYLQESNT KLQEKIDVVP IEPDALFLGL HGRKLCLACV KSGDEIRFQL EAVNITDLSK NKEENKRFTF IRSNSGPTTS FESAACPGWF LCTAQEADRP VSLTNKPKES FMVTKFYLQE DQ.

Product Science Overview

Introduction

Interleukin-1 Receptor Antagonist (IL-1Ra) is a naturally occurring anti-inflammatory protein that plays a crucial role in regulating the immune response by inhibiting the activities of interleukin-1 (IL-1). IL-1 is a pro-inflammatory cytokine involved in various inflammatory and autoimmune conditions. The recombinant form of IL-1Ra, specifically designed for equine use, has shown significant therapeutic potential in veterinary medicine.

Discovery and Development

IL-1Ra was first discovered in 1984 in the urine and serum of patients with leukemia and in the supernatant of cultured monocytes . It was named for its ability to competitively bind to the IL-1 receptor, thereby antagonizing the function of IL-1. The recombinant form of IL-1Ra has been developed to enhance its stability and bioactivity, making it suitable for therapeutic applications.

Mechanism of Action

IL-1Ra functions by binding to the IL-1 receptor on the cell membrane, preventing IL-1 from binding to its receptor and initiating pro-inflammatory signaling pathways . This competitive inhibition helps to reduce inflammation and modulate the immune response. In horses, IL-1Ra has been shown to be effective in treating conditions such as synovitis (inflammation of the joint lining) and mild to moderate osteoarthritis (OA) .

Production and Characterization

Recombinant IL-1Ra can be produced using various expression systems. One study reported the expression and characterization of recombinant IL-1Ra in Aspergillus oryzae, a filamentous fungus . This system offers advantages such as cost-effective production and enhanced stability of the recombinant protein. The recombinant IL-1Ra produced in Aspergillus oryzae was found to be N-glycosylated and exhibited good bioactivity and stability.

Therapeutic Applications in Equine Medicine

The therapeutic potential of IL-1Ra in equine medicine has been extensively studied. IRAP® (Interleukin-1 Receptor Antagonist Protein) therapy, originally developed in Europe, has been widely used in Germany and is now marketed in the United States by Arthrex VetSystems . This therapy is recommended for the treatment of synovitis and mild to moderate osteoarthritis in horses. The use of IL-1Ra in horses helps to reduce inflammation, alleviate pain, and improve joint function.

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