IL 1RA Human His
Greater than 95.0% as determined by SDS-PAGE.
Description
The IL-1Ra His is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Interleukin-1 receptor antagonist (IL-1Ra) is a naturally occurring protein that plays a crucial role in regulating the immune response by inhibiting the activities of interleukin-1 (IL-1). IL-1 is a pro-inflammatory cytokine involved in various inflammatory and immune responses. The recombinant form of IL-1Ra, tagged with a histidine (His) tag, is produced using recombinant DNA technology, allowing for easier purification and enhanced stability.
IL-1Ra was first discovered in 1984 in the urine and serum of patients with leukemia and in the supernatant of cultured monocytes . It is a member of the IL-1 family and binds to IL-1 receptors, blocking the actions of IL-1α and IL-1β . The His tag is a sequence of histidine residues added to the protein to facilitate purification through affinity chromatography.
IL-1Ra functions by competitively binding to IL-1 receptors, thereby preventing IL-1 from exerting its pro-inflammatory effects . This inhibition is crucial in controlling excessive inflammation and immune responses, which can lead to various inflammatory diseases such as rheumatoid arthritis and inflammatory bowel disease.
The recombinant form of IL-1Ra is produced using Escherichia coli or other expression systems. The His tag allows for efficient purification of the protein using nickel-affinity chromatography, which binds to the histidine residues . This method ensures high purity and yield of the recombinant protein.
Recombinant IL-1Ra has been used in clinical settings to treat various inflammatory conditions. It has shown efficacy in reducing inflammation and preventing tissue damage in diseases such as rheumatoid arthritis . Additionally, it has been explored for its potential in treating other conditions involving excessive inflammation, such as sepsis and certain autoimmune diseases.
