IL 18 Mouse, His

Interleukin-18 (IL-18) is a pro-inflammatory cytokine that plays a crucial role in the immune response. It is part of the IL-1 family and is known for its ability to induce the production of interferon-gamma (IFN-γ) in T cells and natural killer (NK) cells. The recombinant form of mouse IL-18, tagged with a His (histidine) tag, is widely used in research to study its functions and interactions.

IL-18 is initially produced as a 24 kDa precursor protein. This precursor is expressed by various cell types, including endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes . The mature form of IL-18 is generated through the cleavage of the precursor by IL-1β converting enzyme (ICE) or caspase-1, resulting in an 18 kDa active protein .

The recombinant mouse IL-18 with a His tag is typically expressed in E. coli. The His tag facilitates the purification of the protein using affinity chromatography techniques. The recombinant protein is often lyophilized and can be reconstituted for experimental use .

IL-18 is known for its ability to enhance the activity of NK cells and stimulate the production of IFN-γ in T-helper type 1 (Th1) cells . This cytokine plays a significant role in both innate and adaptive immunity. In the presence of IL-12 or IL-15, IL-18 enhances antiviral Th1 immune responses by inducing IFN-γ production and the cytolytic activity of CD8+ T cells and NK cells . Conversely, in the absence of IL-12 or IL-15, IL-18 promotes the production of Th2 cytokines such as IL-4 and IL-13 by CD4+ T cells and basophils .

Recombinant mouse IL-18 is used extensively in immunological research to study its role in various physiological and pathological processes. It serves as a valuable tool for investigating the mechanisms of immune responses, inflammation, and autoimmunity. IL-18 has been implicated in several diseases, including rheumatoid arthritis, systemic lupus erythematosus (SLE), and multiple sclerosis .

Lyophilized recombinant mouse IL-18 is stable for up to 12 months when stored at -20 to -80°C. Once reconstituted, the protein solution can be stored at 4-8°C for 2-7 days. For long-term storage, aliquots of the reconstituted protein should be kept at temperatures below -20°C .