IL 18 Human, His

Interleukin-18 Human Recombinant, His Tag
Cat. No.
BT351
Source
Escherichia Coli.
Synonyms
Interferon-gamma-inducing factor, IGIF, IL-1g, IL-18, IL1F4, MGC12320, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, Iboctadekin, IL18.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-18 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 157 amino acids fragment (37-193) having a molecular weight of 20kDa and fused with a 4.5kDa amino-terminal hexahistidine tag.
The IL-18 His is purified by proprietary chromatographic techniques.

Product Specs

Introduction
IL-18, a pro-inflammatory cytokine, stimulates IFN-gamma production in T cells. When combined with IL-12, it can suppress IL-4 dependent IgE and IgG1 production while boosting IgG2a production in B cells. IL-18 Binding Protein (IL18BP) can specifically bind to and negatively regulate IL-18's biological activity.
Description
Recombinant Human Interleukin-18, expressed in E. coli, is a non-glycosylated polypeptide chain comprising amino acids 37-193 of the IL-18 sequence. This 157 amino acid fragment has a molecular weight of 20 kDa and includes a 4.5 kDa N-terminal hexahistidine tag. Purification of IL-18 His is achieved using proprietary chromatographic methods.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
Interleukin-18 His-Tag protein is supplied in a buffer of 20mM Tris-HCl, pH 8.0, with 50% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, freeze at -20°C. Repeated freeze-thaw cycles should be avoided.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Synonyms
Interferon-gamma-inducing factor, IGIF, IL-1g, IL-18, IL1F4, MGC12320, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, Iboctadekin, IL18.
Source
Escherichia Coli.

Product Science Overview

Introduction

Interleukin-18 (IL-18) is a pro-inflammatory cytokine that plays a crucial role in the immune system. It belongs to the IL-1 family and is produced by various cells, including macrophages, dendritic cells, monocytes, neutrophils, and epithelial cells . IL-18 is known for its ability to induce the production of interferon-gamma (IFN-γ) from T cells and natural killer (NK) cells, which is essential for the activation of macrophages and other immune responses .

Structure and Production

Recombinant human IL-18 with a His tag is a form of IL-18 that has been engineered for research purposes. The His tag, a sequence of histidine residues, is added to the protein to facilitate its purification and detection. This recombinant protein is typically expressed in HEK293 cells, a type of human cell line commonly used for protein production .

The recombinant IL-18 protein is produced as a lyophilized powder and is highly pure, with a purity of over 95% as determined by reducing SDS-PAGE . The protein has a calculated molecular weight of 19.3 kDa but is observed to be around 16 kDa due to post-translational modifications .

Biological Functions

IL-18 plays a significant role in both innate and acquired immunity. It facilitates Th1-type immune responses by acting on T cells and stimulating the production of IFN-γ . This cytokine also has roles in various physiological functions, including immunology and metabolic processing .

IL-18 is involved in the regulation of immune responses and has been observed to have a rapid secretion and activation response. It is indicated in various disease conditions, including infectious diseases, inflammation, and autoimmunity . High levels of IL-18 are detected in patients with allergic diseases, autoimmune diseases, and certain chronic inflammatory conditions .

Applications in Research

Recombinant human IL-18 with a His tag is widely used in research to study its biological functions and potential therapeutic applications. It serves as a biomarker or therapeutic target for a wide array of disease indications . Researchers use this recombinant protein to investigate the mechanisms of IL-18 in immune responses and its role in various diseases.

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