IL18BP Human, Sf9
Description
Product Specs
Product Science Overview
Interleukin-18 Binding Protein (IL-18BP) is a naturally occurring inhibitor of the proinflammatory cytokine Interleukin-18 (IL-18). IL-18 is involved in the host’s inflammatory response to infections or injuries and plays a crucial role in regulating both innate and adaptive immune responses . IL-18BP binds to IL-18 with high affinity, effectively neutralizing its activity and inhibiting the inflammatory signaling pathways induced by IL-18 .
IL-18BP was first identified in 1999 as a protein factor that specifically binds to IL-18, antagonizing its biological function . It is a glycoprotein belonging to the immunoglobulin superfamily and is expressed in various tissues and cells in both humans and animals . The human recombinant form of IL-18BP, produced in Sf9 Baculovirus cells, is a single, non-glycosylated polypeptide chain containing 406 amino acids and has a molecular mass of approximately 44.9 kDa .
IL-18BP serves as a natural antagonist of IL-18 by binding to it and preventing its interaction with its receptor. This inhibition results in reduced production of interferon-gamma (IFN-γ) and a decrease in T-helper type 1 immune responses . The protein is constitutively expressed and secreted in mononuclear cells, and elevated levels of IL-18BP have been detected in the intestinal tissues of patients with Crohn’s disease .
The recombinant IL-18BP (Human, Sf9) is produced using Sf9 Baculovirus cells. The protein is purified through proprietary chromatographic techniques to achieve a purity greater than 90% as determined by SDS-PAGE . The recombinant protein is formulated as a sterile filtered colorless solution containing phosphate-buffered saline (pH 7.4) and 10% glycerol .
IL-18BP has significant therapeutic potential due to its ability to inhibit IL-18-induced inflammatory responses. It has been studied for its role in treating various chronic diseases, such as adult-onset Still’s disease and Crohn’s disease . By neutralizing IL-18, IL-18BP can help modulate the progression of these diseases and reduce inflammation .
