IL 15 Mouse

Interleukin-15 Mouse Recombinant
Cat. No.
BT30838
Source
Escherichia Coli.
Synonyms
IL-15, MGC9721, Interleukin-15, Il15, AI503618.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-15 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (49-162 a.a.) and having a molecular mass of 17.6 kDa.
The IL-15 is fused to a 37 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
The cytokine encoded by this gene plays a crucial role in regulating the activation and proliferation of T cells and natural killer cells. This cytokine shares significant biological similarities with interleukin-2 (IL-2), including binding to common hematopoietin receptor subunits. This competition for the same receptor suggests a potential for negative regulation of each other's activity. A key observation is the influence of the balance between this cytokine and IL-2 on the number of CD8+ memory cells. Notably, this cytokine triggers the activation of JAK kinases, leading to the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Research on the mouse counterpart suggests that this cytokine might elevate the expression of the apoptosis inhibitor BCL2L1/BCL-x(L), potentially mediated through STAT6's transcription activation activity, thereby preventing apoptosis. Interestingly, two alternatively spliced transcript variants of this gene have been identified, both encoding the same protein.
Description
Recombinant Mouse Interleukin-15, produced in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 152 amino acids, spanning from positions 49 to 162, and has a molecular weight of 17.6 kDa. For purification purposes, a 37 amino acid His Tag is fused to the N-terminus of IL-15, followed by purification using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The mouse IL-15 protein is supplied in a solution of phosphate-buffered saline (PBS) at a pH of 7.4, supplemented with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the protein should be kept at a refrigerated temperature of 4°C. For extended storage, it is recommended to freeze the protein at -20°C. To further enhance stability during long-term storage, adding a carrier protein like HSA or BSA (0.1%) is advisable. It's important to minimize repeated freeze-thaw cycles to maintain protein integrity.
Purity
Analysis by SDS-PAGE confirms a purity exceeding 90%.
Biological Activity
The biological activity of the protein was assessed using a cell proliferation assay with CTLL2 mouse cytotoxic T cells. The half-maximal effective concentration (ED50) was determined to be less than 1 ng/ml. This translates to a specific activity of 1,000,000 international units (IU) per milligram (mg).
Synonyms
IL-15, MGC9721, Interleukin-15, Il15, AI503618.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMNW IDVRYDLEKI ESLIQSIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILH
EYSNMTLNET VRNVLYLANS TLSSNKNVAE SGCKECEELE EKTFTEFLQS FIRIVQMFIN TS.

Product Science Overview

Introduction

Interleukin-15 (IL-15) is a cytokine that plays a crucial role in the immune system by promoting the proliferation and maintenance of natural killer (NK) cells and CD8+ T cells. It is a member of the four α-helix bundle cytokine family and shares many biological properties with interleukin-2 (IL-2). IL-15 is produced by various cell types, including epithelial cells, monocytes, muscle cells, and placenta cells .

Structure and Function

IL-15 is a potent lymphoid cell growth factor. It stimulates the proliferation of activated T cells and promotes the generation of cytotoxic T lymphocytes (CTLs). Additionally, IL-15 induces the generation, proliferation, and activation of NK cells and supports B cell growth and immunoglobulin production. It is also essential for the maintenance of CD8+ memory T cells .

IL-15 signals through a unique receptor complex that includes the IL-15 receptor α-chain (IL-15Rα) and shares the β- and γ-chains with the IL-2 receptor. This receptor complex allows IL-15 to exert its effects on target cells .

Recombinant Mouse IL-15

Recombinant mouse IL-15 is a laboratory-produced version of the natural cytokine, optimized for use in cell culture, differentiation studies, and functional assays. It is commonly used in research to activate and expand NK and NKT cells, differentiate NK cells from purified CD34+ cells, and expand and activate T cells .

Applications in Research and Therapy

Recombinant mouse IL-15 has several applications in both research and therapeutic contexts:

  1. Activation and Expansion of Immune Cells: IL-15 is used to activate and expand NK and NKT cells, which are critical for immune responses against infections and tumors.
  2. T Cell Differentiation and Activation: IL-15 promotes the differentiation and activation of T cells, including naive CD8+ T cells and CTLs, enhancing their ability to target and eliminate infected or cancerous cells.
  3. Cancer Immunotherapy: IL-15 has shown promise as an immuno-oncology therapeutic due to its ability to stimulate the immune system. It has been used in combination with other therapeutic agents to enhance antitumor activity .

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