IL 15 Human, His
Description
Product Specs
Product Science Overview
Interleukin-15 (IL-15) is a cytokine that plays a crucial role in the regulation of immune responses. It is involved in the activation and proliferation of T cells and natural killer (NK) cells, which are essential components of the immune system . The recombinant form of IL-15, tagged with a histidine (His) tag, is commonly used in research to study its biological functions and potential therapeutic applications.
The IL-15 gene is located on chromosome 4 and encodes a protein that consists of 162 amino acids . The recombinant form of IL-15, produced in Escherichia coli (E. coli), is a non-glycosylated polypeptide chain containing 123 amino acids (fragment 49-162) with a C-terminal hexahistidine tag . This His tag facilitates the purification of the protein using affinity chromatography techniques.
IL-15 shares many biological activities with interleukin-2 (IL-2), including the ability to bind to common hematopoietin receptor subunits . It plays a significant role in the development of inflammatory and protective immune responses by modulating both innate and adaptive immune cells . IL-15 stimulates the proliferation of NK cells, T cells, and B cells, and promotes the secretion of various cytokines .
In monocytes, IL-15 induces the production of chemokines such as IL-8 and monocyte chemotactic protein 1 (CCL2), which attract neutrophils and monocytes to sites of infection . Additionally, IL-15 has been shown to increase the expression of the apoptosis inhibitor BCL2L1/BCL-x(L), potentially preventing apoptosis through the activation of transcription factors such as STAT6 .
Recombinant IL-15 (Human, His Tag) is typically supplied in a sterile filtered solution and formulated in a buffer containing Tris-HCl, PMSF, and glycerol . It should be stored at 4°C if used within 2-4 weeks, or frozen at -20°C for longer periods to maintain its stability . It is important to avoid repeated freeze-thaw cycles to preserve the protein’s activity.
