IL15 Human, HEK

IL-15 is composed of eight exons and is co-expressed with its receptor IL-15Rα, primarily by activated monocytes and dendritic cells (DCs) . The recombinant form of IL-15, expressed in HEK 293 cells, is produced to ensure high purity and biological activity . The recombinant protein is typically lyophilized from a filtered solution containing stabilizers like mannitol or trehalose .

IL-15 shares functional similarities with IL-2, as both cytokines bind to T-cells through the IL-2 receptor β (IL-2Rβ) and IL-2 receptor γ (IL-2Rγ) subunits . However, IL-15 uniquely activates memory-phenotype CD44^hi CD8⁺ cells, mimicking the function of interferon (IFN) by selectively activating these cells . This cytokine is essential for the maintenance and activation of NK cells and multiple T cell subsets, contributing to both humoral and adaptive immune responses .

Recombinant IL-15 has been extensively studied for its potential therapeutic applications, particularly in cancer immunotherapy . It has shown promise in reducing tumor growth in preclinical models by expanding and activating immune cells such as CD8⁺ T cells and NK cells . Additionally, IL-15 is used in various research applications, including cell culture, functional assays, and mass spectrometry .

The recombinant IL-15 protein is typically reconstituted in sterile deionized water and can be stored at 2-8°C for up to one month or at -20°C for extended periods . It is crucial to handle the protein with care to maintain its biological activity and prevent contamination .