IL6 Human, Sf9
BSF2, HGF, HSF, IFNB2, IL-6.
Greater than 95.0% as determined by SDS-PAGE.
Description
IL6 Human Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 189 amino acids (30-212aa) and having a molecular mass of 21.6kDa.(Migrates at 18-28kDa on SDS-PAGE under reducing conditions).
IL6 is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
BSF2, HGF, HSF, IFNB2, IL-6.
VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK ETCNKSNMCE SSKEALAENN
LNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ ARAVQMSTKV
LIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL RQMHHHHHH.
Product Science Overview
Interleukin-6 (IL-6) is a multifunctional cytokine that plays a crucial role in immune regulation, inflammation, and hematopoiesis. It is involved in various physiological processes, including the differentiation of B-cells into immunoglobulin-secreting cells, the growth of myeloma and plasmacytoma cells, and the induction of acute phase reactants in hepatocytes .
Human Recombinant IL-6 is produced using the Sf9 insect cell system, which is derived from the Spodoptera frugiperda (fall armyworm). This system utilizes baculovirus vectors to efficiently express recombinant proteins. The production process involves cloning the human IL-6 gene into a baculovirus transfer vector, which is then co-transfected with linearized baculovirus DNA into Sf9 cells .
The recombinant IL-6 produced in Sf9 cells is a single glycosylated polypeptide chain containing 189 amino acids, with a molecular mass of approximately 21.6 kDa. It is fused to a 6 amino acid His-Tag at the C-terminus and purified using proprietary chromatographic techniques. The protein is typically formulated in a phosphate-buffered saline (PBS) solution with 10% glycerol for stability .
Human Recombinant IL-6 exhibits significant biological activity. It has been shown to induce the proliferation of TF-1 human erythroleukemic cells, with an effective dose (ED50) ranging from 0.2 to 0.8 ng/mL . This activity underscores its importance in various biological processes and its potential therapeutic applications.
The production of Human Recombinant IL-6 using the Sf9 cell system provides a versatile tool for investigating its biological functions and potential therapeutic applications. IL-6 is a target of intensive research due to its pivotal role in immune regulation and inflammation. Understanding its mechanisms can lead to the development of novel therapeutic strategies for various diseases, including autoimmune disorders, cancers, and inflammatory conditions .
