IL36G Human

Interleukin-36 Gamma Human Recombinant
Cat. No.
BT16380
Source
Escherichia Coli.
Synonyms
Interleukin 36 gamma, IL1F9, interleukin 1 family member 9, Interleukin-1 epsilon, IL-1RP2, IL-1H1, IL1E, interleukin 1-related protein 2, Interleukin-1 homolog 1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IL36G Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 169 amino acids and having a molecular mass of 18.7kDa.
The IL36G is purified by proprietary chromatographic techniques.

Product Specs

Introduction
IL-36 gamma (IL-36γ) is a cytokine that belongs to the IL-1 family. This family includes other inflammatory mediators such as IL-1β, IL-1α, IL-1ra, IL-18, IL-36 Ra (IL-1F5), IL-36α (IL-1F6), IL-36β (IL-1F8), IL-37 (IL-1F7), and IL-1F10. These family members share a similar structure, characterized by a 12 β-strand, β-trefoil configuration, and are believed to have originated from a common ancestral gene. IL-36γ is an 18-22 kDa protein composed of 169 amino acids. It exists in both intracellular and secreted forms and lacks a signal sequence, a prosegment, and potential N-linked glycosylation sites. Notably, human IL-36γ exhibits a high degree of amino acid sequence homology with its counterparts in other mammals, ranging from 58% to 69% with mouse, rat, bovine, and equine IL-36γ. However, it shares a lower sequence homology of 23% to 57% with other members of the IL-1 family. The receptor for IL-36γ comprises two subunits: IL-1 Rrp2, which is primarily found on epithelial cells and keratinocytes, and IL-1 RAcP, which is ubiquitously expressed. The binding of IL-36γ to its receptor triggers the activation of downstream signaling pathways, including NF-κB and MAPK pathways, ultimately leading to the production of various inflammatory cytokines and chemokines such as CXCL8/IL-8.
Description
Recombinant human IL-36γ, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 169 amino acids. This protein has a molecular weight of 18.7 kDa and is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder
Formulation
Lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS) at a pH of 7.4 with 5% trehalose.
Solubility
To reconstitute the lyophilized IL-36γ, it is recommended to dissolve it in sterile, ultrapure water (18 MΩ·cm) at a concentration of at least 100 µg/ml. This solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized human IL-36γ is stable at room temperature for up to 3 weeks; however, for long-term storage, it is recommended to store it in a dry environment below -18°C. After reconstitution, IL-36γ should be stored at 4°C for no longer than 2-7 days. For long-term storage, it is advisable to store it below -18°C. It's important to note that repeated freeze-thaw cycles should be avoided.
Purity
The purity is determined to be greater than 95% through the following methods: (a) Analysis by reverse-phase high-performance liquid chromatography (RP-HPLC), (b) Analysis by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Biological Activity
The biological activity of recombinant human IL-36γ is evaluated through its binding affinity to the recombinant human IL-1 Rrp2 Fc chimera protein. This is assessed using a functional enzyme-linked immunosorbent assay (ELISA).
Synonyms
Interleukin 36 gamma, IL1F9, interleukin 1 family member 9, Interleukin-1 epsilon, IL-1RP2, IL-1H1, IL1E, interleukin 1-related protein 2, Interleukin-1 homolog 1.
Source
Escherichia Coli.
Amino Acid Sequence
MRGTPGDADG GGRAVYQSMC KPITGTINDL NQQVWTLQGQ NLVAVPRSDS VTPVTVAVIT CKYPEALEQG RGDPIYLGIQ NPEMCLYCEK VGEQPTLQLK EQKIMDLYGQ PEPVKPFLFY RAKTGRTSTL ESVAFPDWFI ASSKRDQPII LTSELGKSYN TAFELNIND

Product Science Overview

Introduction

Interleukin-36 Gamma (IL-36γ) is a member of the interleukin-1 (IL-1) superfamily, which includes a variety of cytokines involved in inflammatory and immune responses. IL-36γ, along with IL-36α and IL-36β, functions as an agonist, while IL-36Ra acts as an antagonist . These cytokines play crucial roles in modulating the immune system and are implicated in various inflammatory diseases.

Structure and Signaling

IL-36γ is a protein encoded by the IL36G gene located on human chromosome 2 . The IL-36 family members share structural similarities with other IL-1 cytokines, including a conserved β-trefoil fold. IL-36γ binds to the IL-36 receptor (IL-36R), which is a heterodimer composed of IL-1 receptor-related protein 2 (IL-1Rrp2) and the IL-1 receptor accessory protein (IL-1RAcP) . Upon binding to IL-36R, IL-36γ activates downstream signaling pathways, including the nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways .

Biological Functions

IL-36γ is primarily expressed in epithelial cells, keratinocytes, and various immune cells . It plays a significant role in the regulation of immune responses, particularly in the skin, lungs, and gastrointestinal tract. IL-36γ is involved in the activation of immune cells, antigen presentation, and the production of pro-inflammatory cytokines and chemokines . It contributes to host defense mechanisms by promoting inflammation and enhancing the immune response.

Role in Inflammatory Diseases

Dysregulation of IL-36γ has been associated with several inflammatory diseases, including psoriasis, rheumatoid arthritis, and inflammatory bowel disease . In psoriasis, IL-36γ stimulates keratinocytes and immune cells, leading to the activation of the Th17/Th23 axis and the development of psoriatic lesions . Genetic mutations in the IL36RN gene, which encodes the IL-36Ra antagonist, are linked to generalized pustular psoriasis, a severe and life-threatening form of the disease .

Therapeutic Potential

Given its role in inflammatory diseases, IL-36γ has emerged as a potential therapeutic target. Anti-IL-36 antibodies have shown promise in preclinical studies, where they attenuated skin inflammation in mouse models of psoriasis . Further research is needed to explore the therapeutic potential of targeting IL-36γ in other inflammatory conditions.

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