IL36G 152 a.a. Human

Interleukin-36 Gamma (152 a.a) Human Recombinant
Cat. No.
BT16294
Source
Escherichia Coli.
Synonyms
Interleukin 36 gamma, IL1F9, interleukin 1 family member 9, Interleukin-1 epsilon, IL-1RP2, IL-1H1, IL1E, interleukin 1-related protein 2, Interleukin-1 homolog 1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

IL36G (152 a.a.) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 152 amino acids and having a molecular mass of 17.0kDa.
The IL36G (152 a.a.) is purified by proprietary chromatographic techniques.

Product Specs

Introduction
As a member of the IL-1 family, IL-36 gamma plays a crucial role in the inflammatory response. This family, including IL-1b, IL-1a, IL-1ra, IL-18, IL-36 Ra (IL-1F5), IL-36a (IL-1F6), IL-36b (IL-1F8), IL-37 (IL-1F7), and IL-1F10, is believed to share a common ancestral gene and exhibits a characteristic 12 b-strand, b-trefoil structure. IL-36 gamma is an 18-22 kDa protein comprising 169 amino acids. This protein exists in both intracellular and secreted forms and lacks a signal sequence, prosegment, and potential N-linked glycosylation sites. Demonstrating significant homology, human IL-36 gamma shares 58-69% amino acid sequence similarity with its counterparts in mice, rats, bovines, and equines. Furthermore, it exhibits 23-57% sequence homology with other members of the IL-1 family. The IL-36 gamma receptor comprises IL-1 Rrp2, primarily found in epithelial cells and keratinocytes, and the ubiquitously expressed IL-1 RAcP. Activation of the IL-36 receptor by IL-36 isoforms (a, b, and g) triggers the NF-kB and MAPK pathways, a process dependent on IL-1 Rrp2. Notably, IL-36 gamma also stimulates the production of inflammatory mediators such as cytokines and chemokines, including CXCL8/IL-8.
Description
Recombinant human IL36G, specifically a 152-amino acid fragment, is produced in E. coli. This protein is a single, non-glycosylated polypeptide chain with a molecular weight of 17.0 kDa. Purification of IL36G (152 a.a.) is achieved using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized in a sterile solution of 1x PBS (pH 7.4) containing 5% trehalose, filtered through a 0.2 μm filter.
Solubility
For reconstitution, dissolve the lyophilized IL36g in sterile 18M-cm H2O to a concentration of at least 100 μg/ml. The solution can be further diluted in other aqueous solutions.
Stability
Lyophilized IL36g Human is stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store the lyophilized product desiccated at a temperature below -18°C. Once reconstituted, IL36g can be stored at 4°C for 2-7 days. For longer storage durations, store at -18°C. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95.0% based on SDS-PAGE analysis.
Biological Activity
This product exhibits full biological activity comparable to the standard. The ED50, determined by its capacity to stimulate IL-8 secretion in human preadipocytes, is less than 15 ng/ml, equivalent to a specific activity of 67,000 IU/mg.
Synonyms
Interleukin 36 gamma, IL1F9, interleukin 1 family member 9, Interleukin-1 epsilon, IL-1RP2, IL-1H1, IL1E, interleukin 1-related protein 2, Interleukin-1 homolog 1.
Source
Escherichia Coli.
Amino Acid Sequence
SMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND

Product Science Overview

Introduction

Interleukin-36 gamma (IL-36γ), also known as IL-1F9, is a member of the interleukin-1 (IL-1) family of cytokines. This family includes several other cytokines such as IL-1α, IL-1β, IL-18, and IL-36α/β. IL-36γ is a pro-inflammatory cytokine that plays a crucial role in the immune response and inflammation.

Structure and Production

IL-36γ is a protein consisting of 152 amino acids and has a molecular mass of approximately 17.0 kDa . The recombinant form of IL-36γ is typically produced in Escherichia coli (E. coli) and is purified using proprietary chromatographic techniques . The protein is non-glycosylated and exists as a single polypeptide chain.

Function and Signaling

IL-36γ functions by binding to its specific receptor, IL-36R, which is part of the IL-1 receptor family . Upon binding, the IL-36R forms a complex with the IL-1 receptor accessory protein (IL-1RAcP), leading to the activation of intracellular signaling pathways such as NF-κB and MAPK . These pathways are crucial for the transcription of pro-inflammatory genes and the subsequent inflammatory response.

Role in Disease

IL-36γ is primarily expressed in epithelial cells and plays a significant role in skin inflammation. It is involved in the pathogenesis of several inflammatory skin diseases, including psoriasis . Dysregulation of IL-36γ can lead to an enhanced Th17/Th23 axis, contributing to the development of psoriatic-like skin disorders . Genetic mutations in the IL-36 receptor antagonist (IL-36Ra) are associated with generalized pustular psoriasis, a rare but severe skin condition .

Therapeutic Potential

Given its role in inflammation and disease, IL-36γ is a potential target for therapeutic intervention. Anti-IL-36 antibodies have shown promise in attenuating skin inflammation in mouse models of psoriasis . Further research is ongoing to explore the therapeutic potential of targeting IL-36γ in various inflammatory diseases.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

IL36G Human
Interleukin-36 Gamma Human Recombinant
Cat. No.
BT16380
Source
Escherichia Coli.
IL36G Human His
Interleukin-36 Gamma Human Recombinant, His Tag
Cat. No.
BT16482
Source
Escherichia Coli.
IL36G Mouse
Interleukin-36 Gamma Mouse Recombinant
Cat. No.
BT16556
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.