IL36A Human

Interleukin-36 Alpha Human Recombinant
Cat. No.
BT15791
Source
Escherichia Coli.
Synonyms
Interleukin 36 alpha, FIL1E, IL1F6, FIL1, IL1(EPSILON), interleukin 1 family member 6 (epsilon), MGC129552, MGC129553.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IL36A Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 153 amino acids (aa 6-158) and having a molecular mass of 17.0kDa.
The IL36A is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Human IL-36a, a member of the IL-1 family, plays a significant role in inflammatory responses. This family, including IL-1b, IL-1a, IL-1ra, IL-18, IL-36ra (IL1F5), IL-36b (IL1F8), IL-36g (IL1F9), IL-37 (IL1F7), and IL-38 (IL-1F10), is believed to have originated from a common ancestral gene, with members sharing a characteristic 12 β-strand, β-trefoil structure. IL-36a, an 18-22kDa intracellular and secreted protein comprising 158 amino acids, lacks a signal sequence, prosegment, and potential N-linked glycosylation sites. Its release is triggered by stimuli such as LPS and ATP-induced activation of the P2X7 receptor. Notably, human IL-36a exhibits significant amino acid sequence homology with its counterparts in various species, including mouse, rabbit, equine, and bovine (57-68%), and moderate homology with other IL-1 family members (27-57%). Although primarily found in skin and lymphoid tissues, IL-36a is also detected in fetal brain, trachea, stomach, and intestine.
Description
Recombinant Human IL36A, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 153 amino acids (aa 6-158). With a molecular weight of 17.0 kDa, this protein is purified using specialized chromatographic techniques.
Physical Appearance
A sterile, white powder obtained through lyophilization (freeze-drying).
Formulation
Lyophilized from a 0.2 μm filtered solution in 20mM Tris-HCl, 150mM NaCl, 0.1% Tween 20, at a pH of 8.0.
Solubility
To reconstitute the lyophilized IL36A, it is recommended to dissolve it in sterile 18 MΩ-cm H₂O at a concentration of at least 100 μg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Human IL36A is stable at room temperature for up to 3 weeks; however, for long-term storage, it is recommended to store it in a dry environment below -18°C. After reconstitution, store IL36A at 4°C for 2-7 days. For prolonged storage, keep it below -18°C. Avoid repeated freeze-thaw cycles.
Purity
The purity of the product is greater than 95.0%, as determined by: (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
The product exhibits full biological activity comparable to the standard. Its ED50, determined by its capacity to induce IL-8 secretion in human preadipocytes, is less than 10 ng/ml, corresponding to a specific activity of 100,000 IU/mg.
Synonyms
Interleukin 36 alpha, FIL1E, IL1F6, FIL1, IL1(EPSILON), interleukin 1 family member 6 (epsilon), MGC129552, MGC129553.
Source
Escherichia Coli.
Amino Acid Sequence
KIDTPQQGSI QDINHRVWVL QDQTLIAVPR KDRMSPVTIA LISCRHVETL EKDRGNPIYL GLNGLNLCLM CAKVGDQPTL QLKEKDIMDL YNQPEPVKSF LFYHSQSGRN STFESVAFPG WFIAVSSEGG CPLILTQELG KANTTDFGLT MLF

Product Science Overview

Introduction

Interleukin-36 alpha (IL-36α), also known as IL-1F6, is a member of the interleukin-1 (IL-1) family of cytokines. This family includes several other members such as IL-1β, IL-1α, IL-18, IL-36β, IL-36γ, and IL-36Ra. IL-36α plays a crucial role in the regulation of immune responses and inflammation.

Structure and Expression

IL-36α is a protein consisting of 158 amino acids with a molecular weight of approximately 17.2 kDa . It is primarily expressed in epithelial tissues, including the skin, and in various immune cells such as T cells, monocytes, and B cells . The expression of IL-36α is significantly elevated in psoriatic plaques, indicating its involvement in inflammatory skin diseases .

Mechanism of Action

IL-36α functions by binding to its specific receptor, IL-36R (IL-1Rrp2), which is primarily expressed on certain dendritic cells . Upon binding to IL-36R, the receptor complex recruits the IL-1 receptor accessory protein (IL-1RAcP), forming a ternary complex . This complex activates intracellular signaling pathways, including NF-κB and MAPK, leading to the production of pro-inflammatory cytokines and chemokines such as IL-6, IL-8, BD-2, and BD-3 .

Biological Functions

IL-36α is involved in various biological processes, including:

  • Host Defense: It contributes to the host defense by promoting inflammatory responses in epithelial tissues .
  • Skin Inflammation: IL-36α plays a significant role in skin inflammation and is implicated in the pathogenesis of psoriasis . It stimulates keratinocytes and immune cells, enhancing the Th17/Th23 axis and inducing psoriatic-like skin disorders .
  • Other Organs: IL-36α is also expressed in other organs such as the lungs, intestine, joints, and brain, although its physiological and pathological roles in these organs are less well-defined .
Recombinant IL-36α

Recombinant human IL-36α is produced using an E. coli expression system . The recombinant protein is purified to a high degree of purity (≥ 98%) and is used in various research applications to study its biological functions and potential therapeutic uses . It is available in lyophilized form and can be reconstituted for experimental use .

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