IL36A Mouse, His

Interleukin-36 alpha (IL-36α), also known as IL-1F6, is a member of the interleukin-1 (IL-1) family of cytokines. It plays a crucial role in the immune response, particularly in the skin and epithelial tissues. IL-36α is involved in the activation of NF-kappa-B and MAPK signaling pathways, which are essential for generating an inflammatory response . This cytokine is known to participate in the production of other pro-inflammatory cytokines, including IL-12, IL-1β, IL-6, TNF-α, and IL-23 .

Recombinant IL-36α (Mouse, His Tag) is a laboratory-produced version of the natural cytokine, designed for research purposes. It is expressed in Escherichia coli (E. coli) and purified using conventional chromatography techniques . The recombinant protein is fused with a His-tag at the N-terminus, which facilitates its purification and detection.

The preparation of recombinant IL-36α involves several steps:

1. Gene Cloning: The gene encoding IL-36α is cloned into an expression vector, which is then introduced into E. coli cells.
2. Protein Expression: The E. coli cells are cultured under conditions that induce the expression of the recombinant IL-36α protein.
3. Purification: The expressed protein is purified using chromatography techniques, taking advantage of the His-tag for affinity purification .

Recombinant IL-36α is typically analyzed using techniques such as SDS-PAGE and mass spectrometry (MS) to confirm its purity and molecular weight . The protein’s biological activity can be assessed by its ability to activate NF-kappa-B and MAPK signaling pathways in target cells, leading to the production of pro-inflammatory cytokines .

Recombinant IL-36α is used in various research applications, including:

• Studying Inflammatory Responses: Researchers use IL-36α to investigate its role in inflammatory diseases, such as psoriasis and other skin conditions .
• Drug Development: IL-36α is a target for developing new anti-inflammatory drugs.
• Cell Signaling Studies: The protein is used to study the signaling pathways involved in immune responses .

Recombinant IL-36α should be stored at +4°C for short-term use (1-2 weeks) and at -20°C or -70°C for long-term storage. It is important to avoid repeated freezing and thawing cycles to maintain the protein’s stability .