IL1RL1 Human, Sf9

Interleukin-1 Receptor Like-1 Human Recombinant, Sf9
Cat. No.
BT11887
Source
Sf9, Baculovirus cells.
Synonyms
IL33R, Interleukin-1 receptor-like 1, Protein ST2, IL1RL1, DER4, ST2, T1, ST2L, ST2V, FIT-1, MGC32623.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IL 1RL1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (19-328 a.a.) and fused to an 8 aa His Tag at C-terminus containing a total of 318 amino acids and having a molecular mass of 36.0kDa.
IL 1RL1 shows multiple bands between 40-57kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.

Product Specs

Introduction
The IL1RL1 gene, part of the IL-1 receptor family, codes for a transmembrane protein structurally similar to IL-1R1. This protein acts as a receptor for interleukin-33, and upon stimulation, it activates MYD88, IRAK1, IRAK4, and TRAF6. This activation leads to the phosphorylation of MAPK3/ERK1, MAPK1/ERK2, MAPK14, and MAPK8. IL1RL1 might be involved in helper T-cell function and is highly expressed in the kidneys, lungs, placenta, stomach, skeletal muscle, colon, and small intestine. An alternative splicing of the same gene produces a soluble form of IL1RL1, found in various cell types like fibroblasts and mast cells. This soluble form acts as a negative regulator of Th2 cytokine production. Elevated levels of soluble IL1RL1 are linked to several diseases, including asthma, sepsis, and myocardial infarction. Studies on a similar gene in mice suggest that proinflammatory stimuli can induce the IL1RL1 receptor, hinting at its role in helper T-cell function.
Description
IL1RL1, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain containing 318 amino acids (19-328 a.a.). It has an 8 aa His Tag fused at the C-terminus, resulting in a molecular mass of 36.0kDa. On SDS-PAGE under reducing conditions, IL1RL1 displays multiple bands between 40-57kDa. It is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The IL1RL1 protein solution is provided at a concentration of 0.5mg/ml. It is formulated in a buffer consisting of Phosphate buffered saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the IL1RL1 protein solution should be stored at 4°C. For long-term storage, it is recommended to store the solution at -20°C. To enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). It is important to avoid repeated freezing and thawing of the solution.
Purity
The purity of IL1RL1 is determined to be greater than 95.0% using SDS-PAGE analysis.
Synonyms
IL33R, Interleukin-1 receptor-like 1, Protein ST2, IL1RL1, DER4, ST2, T1, ST2L, ST2V, FIT-1, MGC32623.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
KFSKQSWGLE NEALIVRCPR QGKPSYTVDW YYSQTNKSIP TQERNRVFAS GQLLKFLPAA VADSGIYTCI VRSPTFNRTG YANVTIYKKQ SDCNVPDYLM YSTVSGSEKN SKIYCPTIDL YNWTAPLEWF KNCQALQGSR YRAHKSFLVI DNVMTEDAGD YTCKFIHNEN GANYSVTATR SFTVKDEQGF SLFPVIGAPA QNEIKEVEIG KNANLTCSAC FGKGTQFLAA VLWQLNGTKI TDFGEPRIQQ EEGQNQSFSN GLACLDMVLR IADVKEEDLL LQYDCLALNL HGLRRHTVRL SRKNPIDHHS LEHHHHHH.

Product Science Overview

Introduction

Interleukin-1 Receptor Like-1 (IL1RL1), also known as ST2 or IL-33 receptor, is a protein encoded by the IL1RL1 gene. This receptor is a member of the interleukin-1 receptor family and plays a crucial role in both innate and adaptive immunity .

Gene and Protein Structure

The IL1RL1 gene is located on chromosome 2q12 and is part of a cytokine receptor gene cluster that includes IL1R1, IL1R2, and IL1RL2 . The gene encodes a transmembrane protein with a structure similar to IL-1R1. The protein consists of 318 amino acids and has a molecular mass of approximately 36.0 kDa .

Function

IL1RL1 functions as a receptor for interleukin-33 (IL-33). Upon binding to IL-33, IL1RL1 recruits several signaling molecules, including MYD88, IRAK1, IRAK4, and TRAF6. This recruitment leads to the phosphorylation of MAPK3/ERK1, MAPK1/ERK2, MAPK14, and MAPK8, which are involved in various cellular responses . IL1RL1 is implicated in the function of helper T cells and plays a role in tissue homeostasis and responses to environmental stresses .

Expression and Regulation

IL1RL1 is highly expressed in various tissues, including the kidney, lung, placenta, stomach, skeletal muscle, colon, and small intestine . A soluble form of IL1RL1 is produced through alternative splicing and is expressed in several cell types, including fibroblasts and mast cells. This soluble form acts as a negative regulator of Th2 cytokine production and is associated with several disease states, such as asthma, sepsis, and myocardial infarction .

Recombinant Production in Sf9 Cells

The recombinant form of IL1RL1 is produced in Sf9 Baculovirus cells. This recombinant protein is a single, glycosylated polypeptide chain fused to an 8 amino acid His Tag at the C-terminus. The protein is purified using proprietary chromatographic techniques and is available in various quantities for research purposes .

Applications

Recombinant IL1RL1 is used in various research applications, including studies on immune response, inflammation, and disease mechanisms. It is also utilized in the development of therapeutic strategies targeting IL-33/IL1RL1 signaling pathways .

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