IL1RL1 Human, Sf9 Active
Interleukin 1 Receptor Like 1, Homolog Of Mouse Growth Stimulation-Expressed, DER4, ST2, T1, Interleukin 1 Receptor-Related Protein, Interleukin-1 Receptor-Like 1, Growth Stimulation-Expressed, Protein ST2, FIT-1, IL33R, ST2L, ST2V, Interleukin-1 receptor-like 1.
Description
IL1RL1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 318 amino acids (19-328a.a.) and having a molecular mass of 36.0kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
IL1RL1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Recombinant IL1RL1 protein, expressed in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It comprises 318 amino acids (19-328 a.a.), resulting in a molecular mass of 36.0 kDa. Note that the molecular size on SDS-PAGE may appear between 40-57 kDa.
This IL1RL1 protein is engineered with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
The IL1RL1 protein solution is provided at a concentration of 0.5 mg/ml and contains 10% glycerol and Phosphate Buffered Saline (pH 7.4).
To ensure stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended.
Avoid repeated freezing and thawing of the product.
The biological activity of IL1RL1 is assessed by its ability to inhibit the proliferation of D10.G4.1 mouse helper T cells in the presence of IL-33. The ED50 for this inhibitory effect is determined to be less than or equal to 10 ng/ml in the presence of IL-33.
Interleukin 1 Receptor Like 1, Homolog Of Mouse Growth Stimulation-Expressed, DER4, ST2, T1, Interleukin 1 Receptor-Related Protein, Interleukin-1 Receptor-Like 1, Growth Stimulation-Expressed, Protein ST2, FIT-1, IL33R, ST2L, ST2V, Interleukin-1 receptor-like 1.
KFSKQSWGLE NEALIVRCPR QGKPSYTVDW YYSQTNKSIP TQERNRVFAS GQLLKFLPAA VADSGIYTCI VRSPTFNRTG YANVTIYKKQ SDCNVPDYLM YSTVSGSEKN SKIYCPTIDL YNWTAPLEWF KNCQALQGSR YRAHKSFLVI DNVMTEDAGD YTCKFIHNEN GANYSVTATR SFTVKDEQGF SLFPVIGAPA QNEIKEVEIG KNANLTCSAC FGKGTQFLAA VLWQLNGTKI TDFGEPRIQQ EEGQNQSFSN GLACLDMVLR IADVKEEDLL LQYDCLALNL HGLRRHTVRL SRKNPIDHHS LEHHHHHH.
Product Science Overview
Interleukin-1 Receptor Like-1 (IL1RL1), also known as ST2, is a member of the interleukin-1 receptor family. It is a transmembrane protein that plays a crucial role in the immune system by acting as a receptor for interleukin-33 (IL-33). The IL1RL1 gene encodes this protein, which is involved in various immune responses and has been implicated in several diseases.
IL1RL1 is a transmembrane protein with a structure similar to IL-1R1. It is composed of a single, glycosylated polypeptide chain and is fused to an 8 amino acid His Tag at the C-terminus. The protein has a total of 318 amino acids and a molecular mass of approximately 36.0 kDa . When expressed in Sf9 Baculovirus cells, IL1RL1 shows multiple bands between 40-57 kDa on SDS-PAGE under reducing conditions .
IL1RL1 functions as a receptor for IL-33. Upon binding to IL-33, IL1RL1 recruits several signaling molecules, including MYD88, IRAK1, IRAK4, and TRAF6. This recruitment leads to the phosphorylation of MAPK3/ERK1, MAPK1/ERK2, MAPK14, and MAPK8, which are involved in various signaling pathways . IL1RL1 is thought to play a role in helper T-cell function and is highly expressed in tissues such as the kidney, lung, placenta, stomach, skeletal muscle, colon, and small intestine .
IL1RL1 is involved in both innate and adaptive immunity. It contributes to tissue homeostasis and responses to environmental stresses. The receptor is also implicated in the function of helper T cells and may be induced by proinflammatory stimuli . Additionally, IL1RL1 has been shown to act as a negative regulator of Th2 cytokine production. High levels of soluble IL1RL1 have been reported in several disease states, including asthma, sepsis, and myocardial infarction .
Recombinant IL1RL1 produced in Sf9 Baculovirus cells is a valuable tool for research. It is available as a sterile filtered colorless solution and is formulated with phosphate-buffered saline (pH 7.4) and 10% glycerol . The protein is highly pure, with a purity greater than 95.0% as determined by SDS-PAGE . It is recommended to store the protein at 4°C if used within 2-4 weeks, or at -20°C for longer periods. For long-term storage, adding a carrier protein such as 0.1% HSA or BSA is advised to avoid multiple freeze-thaw cycles .
Recombinant IL1RL1 is used in various research applications, including studies on immune responses, signaling pathways, and disease mechanisms. It is particularly useful for investigating the role of IL-33 and its receptor in different physiological and pathological conditions.
