IL1RA Mouse, His Active
Escherichia Coli.
IRAP, IL1F3, IL1RA, IL-1ra3, ICIL-1RA, IL1RN, IL1 inhibitor, IL-1ra, F630041P17Rik.
Greater than 95.0% as determined by SDS-PAGE.
Description
IL1RA Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 177 amino acids (27-178 aa) and having a molecular mass of 20kDa.
IL1RA is fused to a 25 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
IRAP, IL1F3, IL1RA, IL-1ra3, ICIL-1RA, IL1RN, IL1 inhibitor, IL-1ra, F630041P17Rik.
Escherichia Coli.
MGSSHHHHHH SSGLVPRGSH MGSHMRPSGK RPCKMQAFRI WDTNQKTFYL RNNQLIAGYL
QGPNIKLEEK IDMVPIDLHS VFLGIHGGKL CLSCAKSGDD IKLQLEEVNI TDLSKNKEED
KRFTFIRSEK GPTTSFESAA CPGWFLCTTL EADRPVSLTN TPEEPLIVTK FYFQEDQ
Product Science Overview
Interleukin-1 Receptor Antagonist (IL-1Ra) is a naturally occurring anti-inflammatory protein that plays a crucial role in modulating the immune response. It is part of the interleukin-1 (IL-1) cytokine family, which is involved in various inflammatory processes. The recombinant form of IL-1Ra, particularly the mouse version with an active His tag, is widely used in research to study its effects and potential therapeutic applications.
The mouse recombinant IL-1Ra is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain. It consists of 177 amino acids, with a molecular mass of approximately 20 kDa. The protein is fused to a 25 amino acid His tag at the N-terminus, which facilitates its purification through chromatographic techniques .
IL-1Ra functions as an anti-inflammatory antagonist by inhibiting the activities of pro-inflammatory cytokines such as interleukin-1 beta (IL-1β) and interleukin-1 alpha (IL-1α). By binding to the IL-1 receptors without eliciting a signal, IL-1Ra effectively blocks the binding of IL-1α and IL-1β, thereby preventing their pro-inflammatory effects. This regulation is crucial in protecting the body from immune dysregulation and uncontrolled systemic inflammation triggered by various stimulatory agents, including pathogens .
The IL-1 cytokine family, including IL-1Ra, plays a significant role in the initiation and regulation of inflammatory responses. Dysregulation of IL-1 signaling can lead to chronic inflammatory diseases. IL-1Ra, along with other anti-inflammatory members of the IL-1 family such as IL-36Ra, IL-37, and IL-38, is highly expressed in the epidermis and is involved in controlling inflammatory responses in both human and mouse skin .
The recombinant form of IL-1Ra with an active His tag is extensively used in research to study its therapeutic potential in various inflammatory conditions. It is particularly valuable in investigating the mechanisms of immune regulation and the development of anti-inflammatory therapies. The His tag allows for easy purification and detection, making it a useful tool in experimental settings.
