IL1B Canine
Interleukin 1b, Interleukin 1beta, IL-1, IL1F2, IL1-beta, Interleukin 1, beta proprotein, Interleukin 1b, IL1B, interleukin 1 beta, IL-1B.
Sterile filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
IL1B Canine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids (114-265 a.a) and having a molecular mass of 17.5 kDa.
Product Specs
Interleukin-1 beta (IL-1β), also known as IL1B, is a cytokine primarily produced by activated macrophages. It plays a crucial role in stimulating the immune system, including the proliferation of thymocytes (immature T cells) by inducing the release of interleukin-2 (IL-2). IL-1β also contributes to the maturation and proliferation of B cells, enhances fibroblast growth factor activity, and participates in the inflammatory response. As an endogenous pyrogen, IL-1β induces fever and is known to stimulate prostaglandin release from synovial cells.
Recombinant Canine IL-1β, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 153 amino acids (residues 114-265). It has a molecular weight of 17.5 kDa.
The product appears as a sterile, colorless solution that has been filtered for sterilization.
The IL1B Canine protein solution is provided at a concentration of 1 mg/ml in phosphate-buffered saline (PBS) with a pH of 7.4.
The purity of the IL1B Canine protein is greater than 95%, as determined by SDS-PAGE analysis.
The biological activity of the protein was assessed using a cell proliferation assay with D10.G4.1 mouse helper T cells. The ED50 value, representing the effective dose for half-maximal response, is less than or equal to 5 pg/ml.
Interleukin 1b, Interleukin 1beta, IL-1, IL1F2, IL1-beta, Interleukin 1, beta proprotein, Interleukin 1b, IL1B, interleukin 1 beta, IL-1B.
MAAMQSVDCK LQDISHKYLV LSNSYELRAL HLNGENVNKQ VVFHMSFVHG DESNNKIPVV LGIKQKNLYL SCVMKDGKPT LQLEKVDPKV YPKRKMEKRF VFNKIEIKNT VEFESSQYPN WYISTSQVEG MPVFLGNTRG GQDITDFTME FSS.
Product Science Overview
Interleukin-1 beta (IL-1β) is a proinflammatory cytokine that plays a crucial role in the inflammatory response. It is part of the interleukin-1 family, which includes IL-1 alpha (IL-1α) and IL-1β. These cytokines are produced by various cell types, including monocytes, macrophages, and dendritic cells, in response to inflammatory stimuli .
IL-1β is synthesized as an inactive precursor protein with a molecular weight of approximately 31 kDa. This precursor accumulates in the cytosol and is subsequently cleaved by the enzyme caspase-1 to produce the active form of IL-1β, which has a molecular weight of around 17 kDa . The active IL-1β is then secreted from the cell and binds to its receptor, IL-1R1, on target cells to initiate a signaling cascade that leads to the production of various inflammatory mediators .
IL-1β is a key mediator of the inflammatory response and is involved in various physiological and pathological processes. It acts as an endogenous pyrogen, meaning it can induce fever by acting on the hypothalamus . Additionally, IL-1β stimulates the production of prostaglandins, which are lipid compounds that contribute to inflammation and pain . IL-1β also promotes the recruitment of immune cells to sites of infection or injury, thereby enhancing the body’s ability to fight off pathogens and repair damaged tissues .
Recombinant canine IL-1β is a laboratory-produced version of the natural cytokine. It is typically produced using E. coli expression systems, where the gene encoding canine IL-1β is inserted into the bacterial genome, allowing the bacteria to produce the protein . The recombinant protein is then purified and used in various research and clinical applications.
Recombinant canine IL-1β is used in a variety of research settings to study the role of IL-1β in canine health and disease. It is commonly used in cell culture experiments to investigate the effects of IL-1β on canine cells and tissues . Additionally, recombinant IL-1β is used in the development of diagnostic assays, such as ELISA kits, to measure IL-1β levels in biological samples . These assays are valuable tools for studying inflammatory diseases in dogs, including arthritis, epilepsy, and traumatic brain injury .
