IL1A Canine
Sf9, Baculovirus cells.
interleukin 1 alpha, IL1A, interleukin-1 alpha precursor,BAF, Hematopoietin-1, IL1 alpha, IL1F1hematopoietin-1, LAF, LEM, preinterleukin 1 alpha, pro-interleukin-1-alpha.
Greater than 90.0% as determined by SDS-PAGE.
Description
IL1A Canine produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 166 amino acids (109-265 aa) and having a molecular mass of 19.3 kDa.
IL1A is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Interleukin-1 alpha (IL-1 alpha), also known as hematopoietin 1, is a cytokine belonging to the interleukin 1 family. Encoded by the IL1A gene in humans, IL-1 alpha plays a crucial role in inflammation, fever, and sepsis. Its involvement in these processes has led to the development of IL-1 alpha inhibitors for therapeutic purposes. Primarily produced by neutrophils and macrophages, IL-1 alpha is also secreted by endothelial and epithelial cells. By binding to the IL-1 receptor, it significantly contributes to immune response regulation. Furthermore, IL-1 alpha participates in the activation of tumor necrosis factor-alpha (TNF-alpha) and exhibits physiological, metabolic, and hematopoietic functions.
Produced in Sf9 insect cells using a baculovirus expression system, IL1A Canine is a single, glycosylated polypeptide chain. It comprises 166 amino acids (residues 109-265) and has a molecular weight of 19.3 kDa. A 6-amino acid His tag is fused to the C-terminus of IL1A to facilitate purification, which is achieved through proprietary chromatographic techniques.
The IL1A protein is supplied as a solution at a concentration of 0.25 mg/ml. The solution is formulated in Phosphate-Buffered Saline (PBS) with a pH of 7.4 and contains 10% glycerol.
For short-term storage (up to 2-4 weeks), the product should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing of the product should be avoided.
The purity of IL1A Canine is determined by SDS-PAGE analysis and is consistently greater than 90.0%.
The biological activity of IL1A Canine is assessed using a cell proliferation assay with the D10.G4.1 mouse helper T cell line. The ED50 value, which represents the effective concentration for half-maximal response, is typically less than or equal to 2 ng/ml.
interleukin 1 alpha, IL1A, interleukin-1 alpha precursor,BAF, Hematopoietin-1, IL1 alpha, IL1F1hematopoietin-1, LAF, LEM, preinterleukin 1 alpha, pro-interleukin-1-alpha.
Sf9, Baculovirus cells.
ADPSVAYNFH NNEKYNYIRI IKSQFILNDN LNQSIVRQTG GNYLMTAALQ NLDDAVKFDM GAYTSEDSKL PVTLRISKTR LFVSAQNEDE PVLLKEMPET PKTIRDETNL LFFWERHGSK HYFKSVAQPK LFIATQERKL VHMARGQPSI TDFRLLETQP HHHHHH
Product Science Overview
Interleukin-1 alpha (IL-1α) is a cytokine, a type of protein important in cell signaling, that plays a crucial role in the regulation of immune and inflammatory responses. In canines, recombinant IL-1α is used for various research and therapeutic purposes.
IL-1α is a member of the interleukin 1 family, encoded by the IL1A gene. It is produced as a precursor protein that is cleaved to generate the active cytokine. The recombinant form of canine IL-1α is typically produced in Sf9 Baculovirus cells, resulting in a single, glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of approximately 19.3 kDa . This recombinant protein is often fused to a 6 amino acid His tag at the C-terminus for purification purposes .
IL-1α is primarily involved in the regulation of immune responses. It is produced by various cell types, including neutrophils, macrophages, endothelial cells, and epithelial cells . IL-1α binds to the IL-1 receptor, playing a significant role in the activation of tumor necrosis factor-alpha (TNF-α) and other cytokines, which are critical in the body’s response to infection and injury . It is also involved in physiological, metabolic, and hematopoietic activities.
Recombinant IL-1α is used extensively in research to study its role in inflammation, immune response, and disease processes. It is also used in the development of inhibitors that can modulate its activity, which has therapeutic potential for treating inflammatory diseases and conditions associated with excessive immune responses .
The recombinant IL-1α protein is typically stored at 4°C if it will be used within 2-4 weeks. For longer storage, it is recommended to keep it frozen at -20°C, with the addition of a carrier protein such as 0.1% HSA or BSA to prevent degradation . It is important to avoid multiple freeze-thaw cycles to maintain the protein’s stability and activity.
