IL18R1 Human
Interleukin 18 Receptor 1, CD218 Antigen-Like Family Member A, IL1 Receptor-Related Protein, IL-18R-1, CDw218a, IL-18R1, IL-1Rrp, IL1R-Rp, IL1RRP, Interleukin-18 Receptor 1, Cytokine Receptor, CD218a Antigen, IL18Ralpha2, CD218a, IL18RA.
Greater than 90.0% as determined by SDS-PAGE.
Description
IL18R1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing a total of 550 amino acids (19-329 a.a.) and having a molecular mass of 62.7kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). IL18R1 is expressed with a 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
IL18R1, or interleukin 18 receptor 1, belongs to the immunoglobulin superfamily and plays a crucial role in immune regulation. This receptor shares functional similarities with IL12, particularly in regulating immune responses. The expression of IL18R1 on NK and T cells can be induced by IL12 and IFN-alpha. It specifically binds to interleukin 18, a critical step for initiating IL18-mediated signal transduction. While IL18R1 does not interact with IL1A or IL1B beta, it binds to an agonist that activates NF-kappa-B, a key transcription factor involved in immune responses.
Produced in Sf9 Baculovirus cells, IL18R1 is a single, glycosylated polypeptide chain with a molecular weight of 62.7kDa. The protein consists of 550 amino acids, with amino acids 19-329 forming the IL18R1 sequence. A 239 amino acid hIgG-His tag is present at the C-terminus. Purification is achieved through proprietary chromatographic techniques. On SDS-PAGE, IL18R1 appears at a size of approximately 70-100kDa.
The IL18R1 protein solution is provided at a concentration of 0.5mg/ml. It is formulated in a solution containing 10% glycerol and Phosphate buffered saline with a pH of 7.4.
The purity of IL18R1 is determined by SDS-PAGE analysis and is greater than 90.0%.
Interleukin 18 Receptor 1, CD218 Antigen-Like Family Member A, IL1 Receptor-Related Protein, IL-18R-1, CDw218a, IL-18R1, IL-1Rrp, IL1R-Rp, IL1RRP, Interleukin-18 Receptor 1, Cytokine Receptor, CD218a Antigen, IL18Ralpha2, CD218a, IL18RA.
AESCTSRPHI TVVEGEPFYL KHCSCSLAHE IETTTKSWYK SSGSQEHVEL NPRSSSRIAL HDCVLEFWPV ELNDTGSYFF QMKNYTQKWK LNVIRRNKHS CFTERQVTSK IVEVKKFFQI TCENSYYQTL VNSTSLYKNC KKLLLENNKN PTIKKNAEFE DQGYYSCVHF LHHNGKLFNI TKTFNITIVE DRSNIVPVLL GPKLNHVAVE LGKNVRLNCS ALLNEEDVIY WMFGEENGSD PNIHEEKEMR IMTPEGKWHA SKVLRIENIG ESNLNVLYNC TVASTGGTDT KSFILVRKAD MADIPGHVFT RVEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SRDELTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL
SPGKHHHHHH
Product Science Overview
Interleukin-18 (IL-18) is a pro-inflammatory cytokine that plays a crucial role in the immune response. It is involved in the activation of natural killer cells and T-helper 1 (Th1) cells, which are essential for the body’s defense against infections and diseases. The Interleukin-18 receptor (IL-18R) is a heterodimeric receptor composed of two subunits: IL-18 receptor alpha (IL-18Rα) and IL-18 receptor beta (IL-18Rβ). The recombinant form of Interleukin-18 receptor-1 (IL-18R1) is a synthetic version of this receptor, produced using recombinant DNA technology.
IL-18R1 is a membrane-bound receptor that binds to IL-18, initiating a signaling cascade that leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and activator protein 1 (AP-1). These transcription factors are involved in the expression of various genes that mediate inflammatory responses. The receptor consists of an extracellular domain that binds to IL-18, a transmembrane domain, and an intracellular domain that transmits the signal into the cell.
Recombinant IL-18R1 is produced using Escherichia coli (E. coli) as a host organism. The gene encoding IL-18R1 is inserted into a plasmid vector, which is then introduced into E. coli cells. The bacteria are cultured, and the recombinant protein is expressed and purified using chromatographic techniques. The resulting product is a highly pure, biologically active form of IL-18R1 that can be used for research and therapeutic purposes.
Recombinant IL-18R1 is used extensively in research to study the signaling pathways and biological effects of IL-18. It is also used in the development of therapeutic agents for the treatment of inflammatory diseases, such as rheumatoid arthritis and inflammatory bowel disease. By understanding the mechanisms of IL-18 signaling, researchers can develop targeted therapies that modulate the immune response and reduce inflammation.
IL-18 and its receptor play a significant role in various inflammatory and autoimmune diseases. Elevated levels of IL-18 have been associated with conditions such as rheumatoid arthritis, systemic lupus erythematosus, and inflammatory bowel disease. By targeting IL-18R1, researchers aim to develop therapies that can block the interaction between IL-18 and its receptor, thereby reducing inflammation and disease progression.
