IL17F Human, sf9
Cytokine ML-1, IL-17F, Interleukin-17F precursor, IL17F, ML1, ML-1.
Greater than 95.0% as determined by SDS-PAGE.
Description
IL17F produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 142 amino acids (31-163 a.a.) and having a molecular mass of 16kDa (Molecular size on SDS-PAGE will appear at approximately 18-28 kDa). IL17F is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
IL-17F, identified by the accession number Q96PD4, is a cytokine with similarities to IL-17 in its amino acid sequence. Produced by activated T cells, IL-17F has been found to stimulate the production of various other cytokines such as IL6, IL8, and CSF2/GM-CSF. Furthermore, IL-17F demonstrates inhibitory effects on the formation of new blood vessels (angiogenesis) in endothelial cells, while simultaneously promoting these cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. IL-17F also induces stromal cells to produce cytokines involved in inflammation and blood cell formation. Notably, IL-17F gene expression is elevated in the intestines of individuals with active Crohn's disease. Both IL-17A and IL-17F alleles play independent roles in influencing susceptibility to and the pathological characteristics of ulcerative colitis. Genetic variations in IL-17F and MIF genes are significantly linked to the development of functional dyspepsia. The initiation of the IL-17F/IL-17R signaling cascade relies on the ubiquitination of the receptor by TRAF6. IL-17F triggers the expression of IFN-gamma-inducible protein 10 (IP-10) through the activation of the Raf1-mitogen-activated protein kinase 1/2-extracellular-regulated kinase 1/2-p90 ribosomal S6 kinase-cyclic AMP response element-binding protein signaling pathway.
IL17F, produced using Sf9 insect cells infected with a baculovirus expression system, is a single-chain polypeptide that has undergone glycosylation. It consists of 142 amino acids (specifically, amino acids 31 to 163) and has a molecular mass of 16 kDa. When analyzed using SDS-PAGE, it appears as a band in the range of approximately 18-28 kDa. This IL17F protein is engineered with a 9-amino acid Histidine tag located at the C-terminus to facilitate purification. The protein is purified using specialized chromatographic methods.
The IL17F protein solution is supplied at a concentration of 0.5 mg/ml. The solution is formulated in a buffer consisting of Phosphate Buffered Saline (pH 7.4), 20% glycerol, and 1mM DTT.
The purity of the IL17F protein is greater than 95.0% as determined by SDS-PAGE analysis.
Cytokine ML-1, IL-17F, Interleukin-17F precursor, IL17F, ML1, ML-1.
ADPRKIPKVG HTFFQKPESC PPVPGGSMKL DIGIINENQR VSMSRNIESR STSPWNYTVT WDPNRYPSEV VQAQCRNLGC INAQGKEDIS MNSVPIQQET LVVRRKHQGC SVSFQLEKVL VTVGCTCVTP VIHHVQHHHH HH.
Product Science Overview
Interleukin 17F (IL-17F) is a pro-inflammatory cytokine that plays a crucial role in the immune response. It is part of the interleukin 17 family, which includes several other cytokines involved in inflammation and host defense. IL-17F is primarily produced by T helper 17 (Th17) cells, but it can also be secreted by a variety of other cell types, including innate immune cells and epithelial cells .
The IL17F gene is located on chromosome 6p12 in humans . The gene encodes a protein that can form either homodimers or heterodimers with other members of the interleukin 17 family. The recombinant form of IL-17F produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 142 amino acids, with a molecular mass of approximately 16 kDa . This recombinant protein is often tagged with a His tag at the C-terminus to facilitate purification .
IL-17F is involved in the development of inflammation and the host defense against infections. It induces the expression of genes that encode other pro-inflammatory cytokines, such as tumor necrosis factor (TNF), interleukin 1 (IL-1), and interleukin 6 (IL-6) . Additionally, IL-17F promotes the production of chemokines like CXCL1, CXCL5, and interleukin 8 (IL-8), which are essential for neutrophil recruitment and inflammation .
The target cells of IL-17F include epithelial cells, fibroblasts, keratinocytes, synoviocytes, and endothelial cells . By binding to its receptor complex, IL-17RA-IL-17RC, IL-17F triggers signaling pathways that lead to the activation of NF-kappa-B and MAP kinase pathways, resulting in the transcriptional activation of various cytokines, chemokines, antimicrobial peptides, and matrix metalloproteinases .
IL-17F has been implicated in several inflammatory and autoimmune diseases. Elevated levels of IL-17F are often observed in conditions such as psoriasis, rheumatoid arthritis, and inflammatory bowel disease . Due to its role in promoting inflammation, IL-17F is a potential therapeutic target for treating these diseases.
The recombinant form of IL-17F produced in Sf9 Baculovirus cells is used in various research applications to study its biological functions and potential therapeutic uses. This recombinant protein is glycosylated and has a molecular mass of approximately 16 kDa . It is purified using proprietary chromatographic techniques to ensure high purity and activity .
