IL17A Canine
HEK293 cells.
IL17A, IL17, IL-17A, CTLA8, CTLA8, Interleukin 17A, Interleukin-17A, cytotoxic T-lymphocyte-associated serine esterase 8.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
IL17A Canine Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (29-155a.a) containing 133 amino acids and having a molecular mass of 15.6kDa.
IL17A is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Interleukin 17, or IL-17, is a pro-inflammatory cytokine that plays a crucial role in immune responses, particularly in inflammatory diseases. It is primarily produced by activated T helper 17 (Th17) cells and contributes to the recruitment of immune cells and the production of inflammatory mediators.
Canine IL17A Recombinant Protein is a single, glycosylated polypeptide chain produced in HEK293 cells. It consists of 133 amino acids (29-155a.a) and has a molecular weight of 15.6 kDa. The protein is expressed with a 6 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
A clear, colorless solution that has been sterilized through filtration.
The IL17A protein solution has a concentration of 0.5 mg/ml. It is prepared in a solution containing 10% glycerol and Phosphate-Buffered Saline (PBS) at a pH of 7.4.
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. To further enhance stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. It is important to minimize repeated freeze-thaw cycles to maintain protein integrity.
The purity of the protein is determined by SDS-PAGE analysis and is confirmed to be greater than 90%.
The biological activity of IL17A is evaluated using an IL-6 ELISA assay. This assay utilizes NIH/3T3 mouse embryonic fibroblast cells. The ED50 range, which represents the concentration of IL17A that induces half-maximal IL-6 production, is determined to be approximately 10 ng/ml.
IL17A, IL17, IL-17A, CTLA8, CTLA8, Interleukin 17A, Interleukin-17A, cytotoxic T-lymphocyte-associated serine esterase 8.
HEK293 cells.
FPQNPGCRNT EDKNFPQHVK VNLNILNRNT NSRRPSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CVNNEGNINY HMNSVPIQQE ILVLRRESQH CPHSFRLEKM LVAVGCTCVT PIVRHVAHHH HHH
Product Science Overview
IL-17A is a 17 kDa protein that forms a disulfide-linked homodimer. It is primarily produced by a subset of T cells known as Th17 cells. The main function of IL-17A is to stimulate the production of other pro-inflammatory cytokines, such as IL-6, prostaglandin E, and nitric oxide. This leads to the up-regulation of gene expression and the progression and amplification of local inflammation .
Recombinant canine IL-17A has been shown to induce IL-6 secretion in NIH/3T3 mouse embryonic fibroblast cells. The effective dose (ED50) for this activity ranges from 0.3 to 1.5 ng/mL . This biological activity is crucial for understanding the role of IL-17A in various inflammatory conditions and for developing potential therapeutic interventions .
Recombinant canine IL-17A is typically produced in E. coli or HEK293 cells. The protein is then purified using conventional chromatography techniques. The recombinant protein is available in both carrier-free and carrier-containing formulations. The carrier protein, usually Bovine Serum Albumin (BSA), enhances protein stability and shelf-life .
