IL12 Human, Sf9
Baculovirus.
23-219aa (IL12A) / 23-328aa (IL12B), Interleukin 12 (subunit beta/alpha), IL12 His, NKSF1, NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.
Greater than 97.0% as determined by analysis by SDS-PAGE.
Description
Interleukin 12 Human Recombinant is a glycosylated heterodimer containing 503 amino acids (306 amino acid rHuIL-12 p40 and the 197 amino acid rHuIL-12 p35 subunits) and having a molecular mass of 60kDa. IL12 is purified by proprietary chromatographic techniques.
Product Specs
23-219aa (IL12A) / 23-328aa (IL12B), Interleukin 12 (subunit beta/alpha), IL12 His, NKSF1, NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.
Baculovirus.
IL-12 is a heterodimer of IL-12A and IL-12B linked through a disulfide-bond:
>IL-12 ARNLPVATP DPGMFPCLHH SQNLLRAVSN MLQKARQTLE FYPCTSEEID HEDITKDKTS TVEACLPLEL TKNESCLNSR ETSFITNGSC LASRKTSFMM ALCLSSIYED LKMYQVEFKT MNAKLLMDPK RQIFLDQNML AVIDELMQAL NFNSETVPQK SSLEEPDFYK TKIKLCILLH AFRIRAVTID RVMSYLNAS.
>IL-12B
IWELKKDV YVVELDWYPD APGEMVVLTC DTPEEDGITW TLDQSSEVLG SGKTLTIQVK EFGDAGQYTC HKGGEVLSHS LLLLHKKEDG IWSTDILKDQ KEPKNKTFLR CEAKNYSGRF TCWWLTTIST DLTFSVKSSR GSSDPQGVTC GAATLSAERV RGDNKEYEYS VECQEDSACP AAEESLPIEV MVDAVHKLKY ENYTSSFFIR DIIKPDPPKN LQLKPLKNSR QVEVSWEYPD TWSTPHSYFS LTFCVQVQGK SKREKKDRVF TDKTSATVIC RKNASISVRA QDRYYSSSWS EWASVPCS.
Product Science Overview
IL-12 is a heterodimeric cytokine composed of two subunits: IL-12A (p35) and IL-12B (p40). The recombinant human IL-12 produced in Sf9 cells is a glycosylated heterodimer containing 503 amino acids, with a molecular mass of approximately 60 kDa . The two subunits are linked through a disulfide bond, which is essential for its biological activity .
IL-12 is a potent regulator of cell-mediated immune responses. It induces the production of interferon-gamma (IFN-γ) by natural killer (NK) cells and T cells, enhancing their cytotoxic activity . IL-12 also promotes the differentiation of naive T cells into Th1 cells, which are essential for the immune response against intracellular pathogens and tumors .
The recombinant form of IL-12 has been explored for its potential in cancer immunotherapy due to its ability to activate NK cells and cytotoxic T lymphocytes . Clinical trials have investigated the use of IL-12 in treating various cancers, HIV, and hepatitis virus infections . However, the therapeutic application of IL-12 has been limited by its toxicity, particularly the risk of cytokine release syndrome (CRS) .
The recombinant human IL-12 produced in Sf9 cells is purified using proprietary chromatographic techniques to ensure high purity (>97%) . The lyophilized form of IL-12 is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, it should be stored at 4°C for short-term use and below -18°C for future use .
