IL12 Human, His
Interleukin 12 His Tag Human Recombinant
Cat. No.
BT8356
Source
Baculovirus.
Synonyms
23-219aa (IL12A) / 23-328aa (IL12B), Interleukin 12 (subunit beta/alpha), IL12 His, NKSF1, NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
IL12 Human Recombinant produced in a baculovirus expression system is a glycosylated disulfide linked (through cysteines in bold) heterodimer comprised of IL12A (23-219aa, total of 203 aa, MW 23.3kDa) and IL12B (23-328aa, total of 306 aa, MW 34.6kDa), having a total predicted molecular mass of 57.9kDa (Molecular weight on SDS-PAGE will appear higher).
IL12A is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
IL12A is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interleukin-12 (IL-12) is a heterodimeric cytokine that plays a crucial role in the immune system by stimulating the production of interferon gamma (IFN-γ) from T-cells and natural killer (NK) cells. Additionally, IL-12 drives the differentiation of T helper 1 (Th1) cells, establishing its importance in initiating cell-mediated immunity.
Description
Recombinant human IL-12, expressed in a baculovirus system, is a glycosylated heterodimer linked by disulfide bonds. The heterodimer comprises two subunits: IL12A and IL12B. IL12A, with a molecular weight of 23.3 kDa, consists of amino acids 23-219 (totaling 203 amino acids). IL12B, with a molecular weight of 34.6 kDa, is composed of amino acids 23-328 (totaling 306 amino acids). The predicted molecular weight of the complete IL-12 heterodimer is 57.9 kDa. However, the apparent molecular weight on SDS-PAGE may appear higher. For purification, a 6-amino acid His-Tag is fused to the C-terminus of IL12A, and proprietary chromatographic techniques are employed.
Physical Appearance
A clear and colorless solution, devoid of any particulate matter.
Formulation
The IL-12 protein is supplied in a solution containing Phosphate buffered saline (PBS) at a pH of 7.4 and 10% glycerol. The protein concentration is 0.5 mg/ml.
Stability
For short-term storage (2-4 weeks), the IL-12 solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advised for long-term storage. To maintain protein integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of the IL-12 protein is greater than 90% as determined by SDS-PAGE analysis.
Biological Activity
The biological activity of IL-12 is evaluated by measuring its ability to induce IFN-γ production in NK-92 cells using an IFN-γ ELISA. The effective dose that results in 50% activity (ED50) is less than or equal to 0.03 ng/ml.
Synonyms
23-219aa (IL12A) / 23-328aa (IL12B), Interleukin 12 (subunit beta/alpha), IL12 His, NKSF1, NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.
Source
Baculovirus.
Amino Acid Sequence
IL12A(p35):
RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNASHHH HHH.
IL12B(p40):
IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS.
RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNASHHH HHH.
IL12B(p40):
IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS.
Product Science Overview
Introduction
Interleukin 12 (IL-12) is a cytokine that plays a crucial role in the immune system by influencing the activities of T cells and natural killer (NK) cells. It is a heterodimeric protein composed of two subunits: IL-12A (p35) and IL-12B (p40). The recombinant form of IL-12, tagged with a histidine (His) tag, is commonly used in research to study its biological functions and potential therapeutic applications.
Structure and Composition
IL-12 is a disulfide-linked heterodimer consisting of two subunits:
- IL-12A (p35): This subunit has a molecular weight of approximately 23.3 kDa and consists of 203 amino acids .
- IL-12B (p40): This subunit has a molecular weight of approximately 34.6 kDa and consists of 306 amino acids .
The recombinant IL-12 protein is often produced in various expression systems, such as E. coli or baculovirus-infected insect cells, and is tagged with a hexahistidine (His) tag at the N-terminus or C-terminus to facilitate purification and detection .
Biological Functions
IL-12 is a key cytokine in the immune response, with several important functions:
- Activation of T Cells and NK Cells: IL-12 acts as a growth factor for activated T cells and NK cells, enhancing their proliferation and cytotoxic activities .
- Induction of IFN-γ Production: IL-12 stimulates the production of interferon-gamma (IFN-γ) by resting peripheral blood mononuclear cells (PBMCs), which is crucial for the immune response against intracellular pathogens .
- Th1 Cell Differentiation: IL-12 is essential for the differentiation of naive T cells into Th1 cells, which are involved in the defense against intracellular pathogens and in the pathogenesis of autoimmune diseases .
Applications in Research
Recombinant IL-12 with a His tag is widely used in various research applications:
- Immunological Assays: It serves as a positive control in assays such as Western blotting and enzyme-linked immunosorbent assays (ELISA) .
- Functional Studies: Researchers use recombinant IL-12 to study its effects on immune cells and to investigate its potential therapeutic applications in diseases such as cancer and autoimmune disorders .
- Protein-Protein Interactions: The His tag allows for easy purification and detection of IL-12, facilitating studies on its interactions with other proteins and receptors .
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