IL10RA Human

IL-10RA is a subunit of the type II cytokine receptor family. The receptor complex for IL-10 is a heterodimer consisting of IL-10RA and IL-10RB. The IL-10RA subunit is primarily expressed on hematopoietic cells such as B cells, T cells, natural killer (NK) cells, monocytes, and macrophages . It is not typically found in non-hematopoietic cells like fibroblasts or endothelial cells .

The recombinant human IL-10RA protein is often produced in expression hosts such as HEK293 cells or insect cells. The recombinant protein typically includes a polyhistidine tag for purification purposes and is lyophilized for stability .

IL-10 is a pleiotropic cytokine with multiple immunostimulatory and immunosuppressive effects. It regulates immune responses by acting on various cell types, including T cells, B cells, macrophages, and antigen-presenting cells (APCs). IL-10 generally skews the immune response from a TH1 to a TH2 profile, suppressing the production of pro-inflammatory cytokines such as IL-1, IL-6, IL-8, TNF-α, GM-CSF, and G-CSF in activated monocytes and macrophages .

The biological effects of IL-10 are mediated through its binding to the IL-10 receptor complex. Upon binding, the receptor complex activates intracellular signaling pathways, including the JAK-STAT pathway, leading to the phosphorylation of JAK1 and TYK2 kinases .

Recombinant human IL-10RA is used in various research applications to study the immunosuppressive effects of IL-10 and its role in immune regulation. It is also utilized in assays to measure the biological activity of IL-10 by its ability to inhibit IL-10 dependent proliferation of certain cell lines .

Recombinant IL-10RA protein is typically stored in a lyophilized form at -20°C to -80°C. It is stable for up to twelve months under these conditions. After reconstitution, the protein should be stored at 2°C to 8°C for short-term use or at -20°C to -80°C for long-term storage .