IL 10 Mouse
Interleukin-10 Mouse Recombinant
Cat. No.
BT30226
Source
Escherichia Coli.
Synonyms
B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
IL-10 Recombinant Mouse produced in E.coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18785 Dalton.
The Interleukin-10 Mouse is purified by proprietary chromatographic techniques.
The Interleukin-10 Mouse is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interleukin 10 (IL-10) is a cytokine with diverse roles in immune regulation and inflammation, primarily produced by monocytes and to a lesser extent, lymphocytes. It suppresses the immune response by downregulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. Conversely, IL-10 promotes B cell survival, proliferation, and antibody production. It inhibits NF-κB activity and participates in the JAK-STAT signaling pathway. Studies in mice lacking IL-10 have revealed its crucial role in maintaining immune homeostasis in the intestinal tract.
Description
Recombinant Mouse IL-10, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 161 amino acids. With a molecular weight of 18.785 kDa, this purified protein is obtained through proprietary chromatographic techniques.
Physical Appearance
Sterile, white, lyophilized (freeze-dried) powder.
Formulation
The protein was subjected to extensive dialysis against phosphate-buffered saline (PBS) before lyophilization.
Solubility
For reconstitution, it is recommended to dissolve the lyophilized Mouse IL-10 in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Mouse IL-10 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store the desiccated product at temperatures below -18°C. After reconstitution, the recombinant Interleukin-10 should be stored at 4°C for 2-7 days. For prolonged storage, freezing at -18°C is recommended. To enhance stability during storage, the addition of a carrier protein (0.1% HSA or BSA) is advisable. Repeated freeze-thaw cycles should be avoided.
Purity
Purity exceeding 98.0% as determined by: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The ED50, determined by dose-dependent co-stimulation of mouse MC-9 cells with IL-4, was found to be less than 2 ng/ml. This corresponds to a Specific Activity of 500,000 IU/mg.
Synonyms
B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Arg-Gly-Gln.
Product Science Overview
Structure and Production
Interleukin-10 is a member of the IL-10 family of class II cytokines, characterized by a four-helix bundle consisting of α-helical folds . The recombinant form of mouse IL-10 is typically produced in Escherichia coli and is available as a lyophilized powder . It is a single, glycosylated polypeptide chain containing 161 amino acids, with a molecular weight of approximately 18.5 kDa .
Biological Functions
IL-10 is produced by various cell types, including dendritic cells, macrophages, CD4+ T regulatory cells, mast cells, NK cells, neutrophils, and regulatory B cells . It exerts its effects by binding to its receptor, which activates signaling pathways involving JAK1 and STAT3 . The primary functions of IL-10 include:
- Inhibition of Pro-inflammatory Cytokines: IL-10 inhibits the synthesis of cytokines such as IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and helper T-cells .
- Suppression of Immune Cell Activation: It suppresses the activation and proliferation of effector T cells and the production of pro-inflammatory cytokines by neighboring macrophages .
- Promotion of B Cell Function: IL-10 enhances B cell proliferation, immunoglobulin secretion, and class II MHC expression .
- Regulation of Angiogenesis: IL-10 regulates angiogenesis by inducing the expression of either angiogenic or angiostatic factors, depending on the cell type .
Applications
Recombinant mouse IL-10 is widely used in research and clinical applications due to its immunomodulatory properties. Some of the key applications include:
- Cell Culture and Differentiation Studies: IL-10 is used to study the differentiation and function of various immune cells in vitro .
- Functional Assays: It is employed in assays to investigate the biological activities of immune cells and their responses to cytokine stimulation .
- Therapeutic Research: IL-10 is being explored as a potential therapeutic agent for treating inflammatory and autoimmune diseases due to its ability to suppress excessive immune responses .
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