IL10RB Human
Sf9, Insect cells.
Interleukin-10 receptor subunit beta, IL-10 receptor subunit beta, IL-10R subunit beta, IL-10RB, Cytokine receptor class-II member 4, Cytokine receptor family 2 member 4, CRF2-4, Interleukin-10 receptor subunit 2, IL-10R subunit 2, IL-10R2, CDw210b, IL10RB.
Sterile filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
IL10RB Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 440 amino acids (20-220 a.a.) and having a molecular mass of 50.5kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
IL10RB is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Interleukin-10 receptor subunit beta, IL-10 receptor subunit beta, IL-10R subunit beta, IL-10RB, Cytokine receptor class-II member 4, Cytokine receptor family 2 member 4, CRF2-4, Interleukin-10 receptor subunit 2, IL-10R subunit 2, IL-10R2, CDw210b, IL10RB.
Sf9, Insect cells.
MVPPPENVRM NSVNFKNILQ WESPAFAKGN LTFTAQYLSY RIFQDKCMNT TLTECDFSSL SKYGDHTLRV RAEFADEHSD WVNITFCPVD DTIIGPPGMQ VEVLADSLHM RFLAPKIENE YETWTMKNVY NSWTYNVQYW KNGTDEKFQI TPQYDFEVLR NLEPWTTYCV QVRGFLPDRN KAGEWSEPVC EQTTHDETVP SLEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SRDELTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGKHHHHHH.
Product Science Overview
Interleukin-10 receptor beta (IL-10RB), also known as IL-10R2, is a crucial component of the interleukin-10 receptor complex. It plays a significant role in the immune system by mediating the anti-inflammatory effects of interleukin-10 (IL-10). This receptor is part of the cytokine receptor family and is essential for the proper functioning of the immune response.
IL-10RB is a type II cytokine receptor that is ubiquitously expressed in various cell types. It is a shared receptor subunit for several cytokines, including IL-10, IL-22, IL-26, IL-28, and IFN-λ. The receptor complex is tetrameric, consisting of two alpha (IL-10RA) and two beta (IL-10RB) subunits .
The IL-10RB subunit functions as the signaling component of the receptor complex. Upon binding of IL-10 to the IL-10RA subunit, a conformational change occurs in IL-10RB, allowing it to bind to IL-10 as well. This interaction leads to the formation of a heterotetrameric complex that activates the JAK1 and TYK2 kinases . These kinases phosphorylate the IL-10RA subunit, recruiting and phosphorylating STAT3, which then dimerizes and translocates to the nucleus to initiate gene transcription .
Recombinant human IL-10RB is produced using various expression systems, including insect cells and mammalian cells. The recombinant protein is typically glycosylated and has a molecular mass of approximately 50-70 kDa . It is used in research to study the signaling pathways and biological functions of IL-10 and other related cytokines.
IL-10RB is essential for the anti-inflammatory effects of IL-10, which include the inhibition of pro-inflammatory cytokine production and the suppression of antigen presentation. The receptor is also involved in the regulation of gastrointestinal immune responses, particularly in the mucosal layer . Defects in IL-10RB have been associated with early onset inflammatory bowel disease (IBD) and other immune-related disorders .
Mutations in the IL10RB gene can lead to severe immune dysregulation and increased susceptibility to infections and inflammatory diseases. Understanding the structure and function of IL-10RB is crucial for developing therapeutic strategies targeting IL-10 signaling pathways. Recombinant IL-10RB is also used in various assays to study its interactions with other cytokines and to develop potential treatments for immune-related disorders .
