IL34 Human, Sf9
Sf9, Insect cells.
Interleukin-34, IL-34, IL34, C16orf77, Chromosome 16 Open Reading Frame.
Greater than 90% as determined by SDS-PAGE.
Description
IL34 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 231 amino acids (21-242a.a.) and having a molecular mass of 26.3kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
IL34 is expressed with an 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Interleukin-34, IL-34, IL34, C16orf77, Chromosome 16 Open Reading Frame.
Sf9, Insect cells.
ADPNEPLEMW PLTQNEECTV TGFLRDKLQY RSRLQYMKHY FPINYKISVP YEGVFRIANV TRLQRAQVSE RELRYLWVLV SLSATESVQD VLLEGHPSWK YLQEVQTLLL NVQQGLTDVE VSPKVESVLS LLNAPGPNLK LVRPKALLDN CFRVMELLYC SCCKQSSVLN WQDCEVPSPQ SCSPEPSLQY AATQLYPPPP WSPSSPPHST GSVRPVRAQG EGLLPHHHHH H.
Product Science Overview
Interleukin 34 (IL-34) is a cytokine that plays a crucial role in the immune system by acting as a ligand for the colony-stimulating factor-1 receptor (CSF1R). It was first identified in 2008 through comprehensive proteomic analysis and has since been recognized for its significant functions in various biological processes .
IL-34 is a four-helical homodimeric protein that does not belong to any previously identified cytokine family . It is highly conserved across vertebrates, including fish, amphibians, birds, and mammals . The protein is expressed in several tissues, particularly in response to inflammatory stimuli . Notably, IL-34 shares structural homology with macrophage colony-stimulating factor (M-CSF) and signals via the CSF1R .
IL-34 primarily stimulates the proliferation of human peripheral blood monocytes and the formation of macrophage colonies . It promotes the survival of monocytes and the proliferation of macrophages, playing a hematopoietic differentiation role in the bone marrow . Additionally, IL-34 induces the differentiation, adhesion, and proliferation of osteoclasts, contributing to bone homeostasis .
Recombinant IL-34 is produced using various expression systems, including the Sf9 insect cell line. This recombinant protein is optimized for use in cell culture, differentiation studies, and functional assays . It is commonly used in in vitro studies of monocyte, macrophage, and dendritic cell differentiation, as well as in the investigation of CSF1R signaling .
IL-34 has been extensively studied for its role in the development of myeloid compartments, including dendritic cells, monocytes, Langerhans cells, and microglia . Its ability to induce myeloid cell expansion and differentiation makes it a valuable tool in research focused on immune responses and hematopoiesis .
