IL34 Human
CHO cells.
Interleukin 34, C16orf77, MGC34647, IL34
Description
IL34 Human Recombinant produced in CHO cells is a glycosylated homodimeric polypeptide chain containing 228 amino acids and having a total molecular mass of 26kDa.
IL34 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Interleukin 34 (IL34), a cytokine belonging to the interleukin family, plays a crucial role in the immune system. It primarily functions by promoting the differentiation and survival of monocytes and macrophages by interacting with the colony-stimulating factor-1 receptor. This interaction contributes to the growth and survival of these immune cells. IL34's influence extends beyond monocyte regulation, as it is believed to be involved in viral infection responses, adaptive immunity, and bone marrow cell proliferation. Moreover, IL34 is recognized for its significant contribution to innate immunity and inflammatory processes.
Recombinant Human IL34, produced in CHO cells, is a glycosylated homodimeric polypeptide chain with a molecular weight of 26kDa. It consists of 228 amino acids. The protein is engineered with a 6 amino acid His-tag at the C-terminus and undergoes purification using proprietary chromatographic techniques.
The product is lyophilized from a sterile (0.2µm) filtered solution containing phosphate buffered saline (PBS).
For reconstitution, it is recommended to dissolve the lyophilized IL34 in sterile water to a concentration of 0.1 mg/ml. This solution can be further diluted in other aqueous solutions as needed.
Lyophilized IL34 demonstrates stability at room temperature for up to 3 weeks. However, for optimal storage, it is recommended to store the lyophilized product desiccated below -18°C. After reconstitution, IL34 Human should be stored at 4°C for a period of 2-7 days. For long-term storage, it is advisable to store the reconstituted product below -18°C. The addition of a carrier protein, such as 0.1% HSA or BSA, is recommended for long-term storage. It's important to avoid repeated freeze-thaw cycles.
Interleukin 34, C16orf77, MGC34647, IL34
CHO cells.
NEPLEMWPLT QNEECTVTGF LRDKLQYRSR LQYMKHYFPI NYKISVPYEG VFRIANVTRLQRAQVSEREL RYLWVLVSLS ATESVQDVLL EGHPSWKYLQ EVETLLLNVQ QGLTDVEVSPKVESVLSLLN APGPNLKLVR PKALLDNCFR VMELLYCSCC KQSSVLNWQD CEVPSPQSCSPEPSLQYAAT QLYPPPPWSP SSPPHSTGSV RPVRAQGEGL LPHHHHHH
Product Science Overview
Interleukin-34 (IL-34) is a cytokine that plays a crucial role in the regulation of immune and inflammatory responses. It is a homodimeric protein that does not belong to any previously identified cytokine family. IL-34 is expressed by various tissues in response to inflammatory stimuli and has significant implications in hematopoiesis, immune response, and cellular differentiation.
IL-34 is a four-helical homodimeric protein. The human IL-34 protein is synthesized as a 242 amino acid precursor, which includes a 20 amino acid signal sequence and a 222 amino acid mature chain . It shares structural homology with macrophage colony-stimulating factor (M-CSF) and signals through the colony-stimulating factor receptor 1 (CSFR1) . This signaling pathway is essential for the expansion and differentiation of myeloid cells.
IL-34 has several critical biological functions:
- Monocyte and Macrophage Survival: IL-34 promotes the survival of monocytes and the proliferation of macrophages .
- Hematopoietic Differentiation: It plays a role in hematopoietic differentiation in the bone marrow, inducing colony-forming units of macrophages and promoting osteoclast differentiation, adhesion, and proliferation .
- Immune Response: IL-34 is involved in the development of myeloid compartments, including dendritic cells, monocytes, Langerhans cells, and microglia .
Recombinant human IL-34 is produced using various expression systems, including HEK293 cells and mouse myeloma cell lines . It is optimized for use in cell culture, differentiation studies, and functional assays. The recombinant protein is typically purified to a high degree, with endotoxin levels below 0.1 ng/μg and purities greater than 95% .
Recombinant IL-34 has several applications in research and clinical studies:
- Cell Culture: It is used in vitro studies of monocyte, macrophage, and dendritic cell differentiation .
- Developmental Studies: IL-34 is utilized in developmental studies of bone marrow and the central nervous system .
- CSFR1 Signaling: It is employed in the investigation of CSFR1 signaling pathways .
