IL 13 Mouse
Interleukin-13 Mouse Recombinant
Cat. No.
BT30655
Source
Escherichia Coli.
Synonyms
Interleukin-13, NC300, ALRH, BHR1, P600, IL-13, IL13.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Interleukin-13 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 111 amino acids and having a molecular mass of 12.3 kDa.
The IL-13 is purified by proprietary chromatographic techniques.
The IL-13 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interleukin 13 (IL-13) is a cytokine primarily secreted by activated Th2 cells, playing a crucial role in regulating immune responses. IL-13 is involved in multiple stages of B cell development, including maturation and differentiation. It enhances the expression of CD23 and MHC class II molecules on B cells, facilitating their activation and antigen presentation. Moreover, IL-13 promotes immunoglobulin class switching to IgE, a process crucial for allergic responses. Conversely, IL-13 exerts suppressive effects on macrophages, leading to a reduction in the production of pro-inflammatory cytokines and chemokines, thereby dampening excessive inflammation. While IL-13 is recognized as a critical mediator in allergen-induced asthma, its underlying mechanisms extend beyond its interactions with IgE and eosinophils. Notably, the genes encoding IL-13, IL3, IL5, IL4, and CSF2 are clustered together on chromosome 5q, with IL-13 positioned closely to IL4, suggesting potential functional relationships and coordinated regulation.
Description
Recombinant Mouse Interleukin-13, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 111 amino acids. With a molecular weight of 12.3 kDa, this protein is purified using proprietary chromatographic techniques to ensure high purity and activity.
Physical Appearance
Sterile White Lyophilized Powder
Formulation
The lyophilized Interleukin-13 protein is provided at a concentration of 1 mg/ml in a buffer consisting of PBS (pH 7.2) and 5% trehalose.
Solubility
To reconstitute lyophilized Interleukin-13, it is recommended to dissolve the protein in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Interleukin-13 exhibits stability at room temperature for a period of 3 weeks. However, for long-term storage, it is recommended to store the lyophilized protein in a desiccated state below -18°C. After reconstitution, Interleukin-13 should be stored at 4°C for up to 7 days. For extended storage, it is advisable to add a carrier protein such as HSA or BSA (0.1%) to the solution and store it below -18°C. Repeated freeze-thaw cycles should be avoided to maintain protein integrity and activity.
Purity
The purity of Interleukin-13 is determined to be greater than 95% using SDS-PAGE analysis.
Biological Activity
The biological activity of Interleukin-13 is measured by its ability to induce the proliferation of TF-1 cells. The ED50, representing the concentration at which 50% of the maximal proliferative response is observed, falls within the range of 4 ng/ml. This corresponds to a specific activity of 250,000 IU/mg.
Protein Content
The protein content of Interleukin-13 is quantified using two independent methods: 1) UV spectroscopy at 280 nm, employing an extinction coefficient of 0.69 for a 0.1% (1 mg/ml) solution, calculated using the PC GENE computer analysis program; and 2) Reverse-phase high-performance liquid chromatography (RP-HPLC), utilizing a calibrated solution of Interleukin-13 as a reference standard.
Synonyms
Interleukin-13, NC300, ALRH, BHR1, P600, IL-13, IL13.
Source
Escherichia Coli.
Amino Acid Sequence
MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F.
Product Science Overview
Introduction
Interleukin-13 (IL-13) is a cytokine, a type of protein important in cell signaling, that plays a crucial role in the immune system. It is primarily produced by T helper type 2 (Th2) cells, CD4 cells, natural killer T cells, mast cells, basophils, eosinophils, and nuocytes . IL-13 is involved in various biological processes, including the regulation of immune responses, inflammation, and cell differentiation.
Structure and Function
IL-13 is a protein that, in mice, is encoded by the IL13 gene located on chromosome 11 . The protein has a molecular mass of approximately 12.3 kDa and consists of 111 amino acids . It folds into four alpha-helical bundles, a structural feature similar to that of Interleukin-4 (IL-4), although IL-13 only has 25% sequence identity with IL-4 .
IL-13 is a central regulator in several immune processes, including:
- IgE synthesis: IL-13 promotes the production of Immunoglobulin E (IgE), which is involved in allergic reactions .
- Goblet cell hyperplasia and mucus hypersecretion: It induces the proliferation of goblet cells and increases mucus production, which is significant in conditions like asthma .
- Airway hyperresponsiveness: IL-13 contributes to the increased sensitivity of the airways, a hallmark of asthma .
- Fibrosis: It plays a role in tissue remodeling and fibrosis, which can occur in chronic inflammatory conditions .
Recombinant IL-13
Recombinant IL-13 refers to the IL-13 protein that is produced using recombinant DNA technology. This involves inserting the IL13 gene into a suitable expression system, such as E. coli, to produce the protein in large quantities . Recombinant IL-13 is used in various research applications, including cell culture, differentiation studies, and functional assays .
Applications in Research
Recombinant IL-13 is widely used in scientific research to study its role in immune responses and disease mechanisms. Some key applications include:
- Cell culture: Recombinant IL-13 is used to stimulate cells in culture to study their responses to this cytokine .
- Differentiation studies: Researchers use recombinant IL-13 to investigate its effects on the differentiation of various cell types, such as B cells and macrophages .
- Functional assays: It is employed in assays to measure its biological activity, such as its ability to promote cell proliferation or cytokine production .
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