IL 13 Rat 109 a.a.

Interleukin-13 109 a.a. Rat Recombinant
Cat. No.
BT30736
Source
Escherichia Coli.
Synonyms
NC300, ALRH, BHR1, P600, IL-13.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Interleukin-13 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 109 amino acids and having a molecular mass of 11.9 kDa.
The IL-13 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
IL-13, primarily produced by activated Th2 cells, is an immunoregulatory cytokine with a key role in B-cell maturation and differentiation. It increases CD23 and MHC class II expression while promoting IgE isotype switching in B cells. Additionally, IL-13 suppresses macrophage activity, thereby inhibiting pro-inflammatory cytokines and chemokines. Playing a crucial role in allergen-induced asthma, IL-13 functions independently of IgE and eosinophils. This gene is part of a cytokine gene cluster on chromosome 5q, located near IL3, IL5, IL4, and CSF2, with a particularly close proximity to IL4.
Description
Recombinant Rat Interleukin-13, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 109 amino acids. With a molecular weight of 11.9 kDa, this IL-13 is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein was lyophilized at a concentration of 1 mg/ml in phosphate-buffered saline (PBS) at pH 7.4.
Solubility
To reconstitute the lyophilized Interleukin-13, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Interleukin-13 remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated below -18°C. After reconstitution, IL-13 should be stored at 4°C for 2-7 days. For long-term storage, freezing below -18°C is recommended. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95% using SDS-PAGE analysis.
Biological Activity
The ED50 range is determined to be 40 ng/ml, which corresponds to a specific activity greater than 25,000 IU/mg. This was determined by measuring the dose-dependent proliferation of human TF-1 cells. The optimal concentration for specific applications should be determined through a dose-response assay.
Protein Content
The protein content is quantified using two independent methods: 1) UV spectroscopy at 280 nm, utilizing an absorbance value of 0.69 as the extinction coefficient for a 0.1% (1 mg/ml) solution, calculated using the PC GENE computer analysis program (IntelliGenetics), and 2) Analysis by RP-HPLC, employing a calibrated solution of IL-13 as a reference standard.
Synonyms
NC300, ALRH, BHR1, P600, IL-13.
Source
Escherichia Coli.
Amino Acid Sequence
VRRSTSPPVA LRELIEELSN ITQDQKTSLC NSSIVWSVDI TAGGFCAALE SLTNISSCNA IHRTQRILNG LCNQKASDVA SSPPDTKIEV AQFISKLLNY SKQLFRYGH.

Product Science Overview

Structure and Production

Recombinant rat IL-13 is a single, non-glycosylated polypeptide chain with a molecular mass of approximately 11.9 kDa . It is produced in Escherichia coli (E. coli) expression systems, ensuring high purity and consistency. The amino acid sequence of rat IL-13 is as follows:

VRRSTSPPVA LRELIEELSN ITQDQKTSLC NSSIVWSVDI TAGGFCAALE SLTNISSCNA IHRTQRILNG LCNQKASDVA SSPPDTKIEV AQFISKLLNY SKQLFRYGH
Biological Functions

IL-13 is involved in various stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression and promotes IgE isotype switching of B cells . Additionally, IL-13 down-regulates macrophage activity, inhibiting the production of pro-inflammatory cytokines and chemokines. This cytokine is critical in the pathogenesis of allergen-induced asthma, operating through mechanisms independent of IgE and eosinophils .

Applications in Research

Recombinant rat IL-13 is widely used in research to study its role in immune regulation and inflammatory responses. It is particularly valuable in:

  • Allergy and Asthma Research: Investigating the mechanisms of allergen-induced asthma and the role of IL-13 in these processes.
  • B-cell Biology: Understanding the stages of B-cell maturation, differentiation, and isotype switching.
  • Macrophage Activity: Exploring the inhibitory effects of IL-13 on macrophage activity and its impact on pro-inflammatory cytokine production.
Storage and Handling

The lyophilized form of recombinant rat IL-13 is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use. It is recommended to add a carrier protein (0.1% HSA or BSA) to prevent freeze-thaw cycles .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

IL13RA1 Human
Interleukin 13 Receptor Alpha 1 Human Recombinant
Cat. No.
BT8782
Source
E.coli.
IL 13 Human, His
Interleukin-13 Human Recombinant, His Tag
Cat. No.
BT30622
Source
Escherichia Coli.
IL 13 Rat
Interleukin-13 Rat Recombinant
Cat. No.
BT30691
Source
Escherichia Coli.
IL 13 Variant Human
Interleukin-13 Variant Human Recombinant
Cat. No.
BT30794
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.