IL 13 Rat
Interleukin-13 Rat Recombinant
Cat. No.
BT30691
Source
Escherichia Coli.
Synonyms
NC300, ALRH, BHR1, P600, IL-13.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Interleukin-13 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 113 amino acids and having a molecular mass of 12.7 kDa.
The IL-13 is purified by proprietary chromatographic techniques.
The IL-13 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interleukin 13 (IL-13) is a cytokine secreted by activated T helper type 2 (Th2) cells. It plays a crucial role in the immune response, particularly in allergic and inflammatory reactions. IL-13 is involved in various biological processes, including B cell maturation, IgE antibody production, and the regulation of inflammatory responses. It exerts its effects by binding to specific receptors on target cells, leading to the activation of downstream signaling pathways and gene expression.
Description
Recombinant Rat Interleukin-13, expressed in E. coli, is a purified protein with a molecular weight of approximately 12.7 kDa. It consists of a single polypeptide chain comprising 113 amino acids. The protein is produced as a non-glycosylated form, lacking carbohydrate modifications.
Physical Appearance
The product appears as a sterile, white powder obtained through lyophilization (freeze-drying).
Formulation
The lyophilized Interleukin-13 is provided at a concentration of 1 mg/ml and does not contain any additives or excipients.
Solubility
To reconstitute the lyophilized Interleukin-13, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a minimum concentration of 100 µg/ml. The reconstituted solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Interleukin-13 is stable at room temperature for up to 3 weeks. However, it is recommended to store the desiccated product at -18°C or below for optimal long-term stability. After reconstitution, IL-13 should be stored at 4°C for 2-7 days. For extended storage, freezing at -18°C or below is advised, and the addition of a carrier protein like HSA (human serum albumin) or BSA (bovine serum albumin) at a concentration of 0.1% is recommended. Repeated freeze-thaw cycles should be avoided to maintain protein integrity.
Purity
The purity of Interleukin-13 is determined using SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and is found to be greater than 95%.
Biological Activity
The biological activity of Interleukin-13 is measured by its ability to stimulate the proliferation of human TF-1 cells. The ED50 (half-maximal effective dose) range is determined to be between 2 and 6 ng/ml. It is important to note that the optimal concentration for specific applications may vary and should be determined through appropriate dose-response assays.
Protein Content
The protein concentration of Interleukin-13 is quantified using two independent methods: 1) UV spectroscopy at 280 nm, employing an extinction coefficient of 0.69 for a 0.1% (1 mg/ml) solution, as calculated by the PC GENE computer analysis program (IntelliGenetics), and 2) RP-HPLC (reverse-phase high-performance liquid chromatography) analysis, utilizing a calibrated solution of IL-13 as a reference standard.
Synonyms
NC300, ALRH, BHR1, P600, IL-13.
Source
Escherichia Coli.
Product Science Overview
Structure and Function
IL-13 is a monomeric protein that circulates with two internal disulfide bonds, contributing to a bundled four alpha-helix configuration . The mature rat IL-13 shares 59%, 75%, and 60% amino acid sequence identity with human, mouse, and rhesus IL-13, respectively . This structural similarity allows for cross-species studies and applications.
IL-13 is involved in various biological processes, including:
- Regulation of immune responses: It modulates the activity of immune cells, influencing the production of other cytokines and the differentiation of T cells.
- Inflammation: IL-13 plays a role in allergic inflammation and is implicated in conditions such as asthma and allergic rhinitis.
- Tissue remodeling: It contributes to tissue repair and fibrosis by promoting the production of extracellular matrix proteins.
Recombinant Production
Applications
Recombinant IL-13 is utilized in several research areas:
- Cell proliferation assays: It is used to measure the proliferation of specific cell lines, such as TF-1 human erythroleukemic cells .
- Immunological studies: Researchers use recombinant IL-13 to investigate its role in immune regulation and inflammatory responses.
- Disease models: It is employed in animal models to study diseases like asthma, fibrosis, and other inflammatory conditions.
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