IGF1 Rabbit

Insulin-Like Growth Factor 1 Rabbit Recombinant
Cat. No.
BT18408
Source
Escherichia Coli. 
Synonyms
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0%  as determined by:
(a) Gel-filtration chromatography under non denaturing conditions.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Insulin-Like Growth Factor-I Rabbit Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7639 Dalton. 

Product Specs

Introduction
The IGF family of peptides plays a crucial role in the growth and development of mammals. IGF1 is a key mediator of growth hormone's growth-promoting effects. Research has shown that growth hormone doesn't directly stimulate sulfate incorporation into cartilage. Instead, it acts through a serum factor initially called 'sulfation factor' and later renamed 'somatomedin'. Three primary somatomedins have been identified: somatomedin C (IGF1), somatomedin A, and somatomedin B. Primarily, but not exclusively, produced by the liver, IGF-1 is a small protein that circulates in the blood mainly in a complex with several IGF binding proteins. It exhibits growth-regulating, insulin-like, and mitogenic properties and is secreted in response to growth hormone stimulation.
Description
Recombinant Insulin-Like Growth Factor-I, produced in E. Coli using rabbit genes, is a single, non-glycosylated polypeptide chain consisting of 71 amino acids. It has a molecular weight of 7639 Daltons.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein solution, at a concentration of 0.5mg/ml in 0.02% NaHCO3, was lyophilized.
Solubility

For reconstitution of lyophilized rbIGF-I, it is recommended to use sterile 0.4% NaHCO3 adjusted to a pH of 8-9. The initial reconstitution should be at a concentration no less than 100 μg/ml. This solution can be further diluted with other aqueous solutions as needed.

Stability
Lyophilized Insulin-Like Growth Factor-1 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it should be stored in a desiccated state at a temperature below -18°C. After reconstitution, IGF1 can be stored at 4°C for 2-7 days. For extended storage, it should be kept at -18°C. Adding a carrier protein such as 0.1% HSA or BSA is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 98.0% using the following methods:
(a) Gel-filtration chromatography under non-denaturing conditions.
(b) SDS-PAGE analysis.
Biological Activity
The biological activity of IGF-I is comparable to human IGF-1. The ED50, determined by the dose-dependent proliferation of human MCF/7 cells, ranges from 5 to 25 ng/ml in the cell culture mixture. This variation depends on specific culture conditions. The activity of rbIGF-I is approximately 30-40% of that observed with human IGF-1.
Synonyms
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.
Source
Escherichia Coli. 
Amino Acid Sequence
MTAGPETLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKP

Product Science Overview

Structure and Function

IGF-1 is a small protein with a molecular weight of approximately 7.65 kDa . It interacts with the IGF-1 receptor and the insulin receptor to regulate cell growth, differentiation, and apoptosis. This interaction is essential for normal growth and development, particularly during childhood and adolescence .

Recombinant IGF-1 Production

Recombinant IGF-1 is produced using various expression systems, including E. coli, yeast, and mammalian cells. The recombinant form of IGF-1 is used in research and therapeutic applications due to its ability to mimic the biological activity of natural IGF-1. The production process involves cloning the IGF-1 gene into an expression vector, transforming the host cells, and purifying the expressed protein .

Applications in Research and Medicine

Recombinant IGF-1 is widely used in scientific research to study its effects on cell growth, metabolism, and aging. It is also used in the development of treatments for conditions such as growth hormone insensitivity, muscle wasting, and certain types of cancer .

In rabbits, IGF-1 has been shown to play a significant role in fetal development and postnatal growth. Studies have demonstrated that IGF-1 levels are crucial for normal development and that recombinant IGF-1 can be used to study various physiological processes in rabbits .

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