IGF1 Human, R36Q

Insulin Like Growth Factor-1, Mutant R36Q Human Recombinant
Cat. No.
BT17971
Source
Escherichia Coli.
Synonyms
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

IGF1 R36Q Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of Approximately 7.7kDa.

The IGF1 R36Q is purified by proprietary chromatographic techniques.

Product Specs

Introduction
The somatomedins, also known as insulin-like growth factors (IGFs), are peptides involved in mammalian growth and development. IGF1 plays a crucial role in mediating the growth-promoting effects of growth hormone (GH). Studies have shown that GH does not directly stimulate sulfate incorporation into cartilage but acts through a serum factor originally called 'sulfation factor' and later identified as 'somatomedin'. Three main somatomedins have been identified: somatomedin C (IGF1), somatomedin A (IGF2), and somatomedin B.
Description
IGF1 R36Q Human Recombinant, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 70 amino acids. It has a molecular weight of approximately 7.7 kDa. The purification of IGF1 R36Q is carried out using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
IGF1 R36Q is lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB and 150 mM NaCl, pH 5.4.
Solubility
For reconstitution, it is recommended to dissolve the lyophilized IGF1 R36Q in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. Further dilutions can be made in other aqueous solutions.
Stability
For short-term storage (2-4 weeks), store the vial at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Synonyms
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.
Source
Escherichia Coli.
Amino Acid Sequence

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSQRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.

Product Science Overview

Introduction

Insulin-Like Growth Factor-1 (IGF-1) is a peptide hormone that plays a crucial role in growth and development in mammals. It is structurally similar to insulin and is involved in cellular growth, differentiation, and survival. IGF-1 is produced primarily in the liver and is regulated by growth hormone (GH). The mutant R36Q variant of IGF-1 is a specific alteration where the arginine ® at position 36 is replaced by glutamine (Q). This mutation can affect the biological activity and binding properties of IGF-1.

Biological Significance

IGF-1 mediates many of the growth-promoting effects of GH. It is involved in various physiological processes, including:

  • Cellular proliferation: IGF-1 stimulates the growth and division of cells.
  • Differentiation: It promotes the maturation of cells into specialized cell types.
  • Survival: IGF-1 has anti-apoptotic properties, helping cells to survive under stress conditions.
Recombinant Production

Recombinant human IGF-1 (rhIGF-1) is produced using genetic engineering techniques. The gene encoding IGF-1 is inserted into a suitable expression system, such as bacteria or yeast, which then produces the protein. The recombinant protein is purified and used for various research and therapeutic purposes.

Mutant R36Q Variant

The R36Q mutation in IGF-1 can alter its interaction with IGF-1 receptors and binding proteins. This mutation may affect the stability, affinity, and overall biological activity of the protein. Studies on the R36Q variant help in understanding the structure-function relationship of IGF-1 and its role in different physiological and pathological conditions.

Applications and Research

Recombinant IGF-1, including the R36Q mutant, is used in various research applications:

  • Diabetes Research: IGF-1 has been studied for its potential to improve insulin sensitivity and glycemic control in patients with type 2 diabetes .
  • Growth Disorders: It is used in the treatment of growth hormone insensitivity syndromes and other growth-related disorders.
  • Cancer Research: IGF-1 signaling pathways are investigated for their role in cancer cell proliferation and survival.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

IGF1 Gilthead Seabream
IGF1 Gilthead Seabream Recombinant
Cat. No.
BT17466
Source
Escherichia Coli.
IGF1 Human
IGF-1 Human Recombinant
Cat. No.
BT17540
Source
Escherichia Coli. 
IGF1 Mouse
Insulin-Like Growth Factor 1 Mouse Recombinant
Cat. No.
BT18175
Source
Escherichia Coli.
LR3 IGF1 Human
LR3 Insulin Like Growth Factor-1 Human Recombinant
Cat. No.
BT18739
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.