IGF1 Human, A70T

Insulin Like Growth Factor-1, Mutant A70T Human Recombinant
Cat. No.
BT17776
Source
Escherichia Coli.
Synonyms
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IGF1 A70T Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of Approximately 7.7kDa.

The IGF1 A70T is purified by proprietary chromatographic techniques.

Product Specs

Introduction
The somatomedins, also known as insulin-like growth factors (IGFs), are a family of peptides that play a crucial role in mammalian growth and development. IGF1 is a key mediator of the growth-promoting effects of growth hormone (GH). Early research revealed that growth hormone did not directly stimulate sulfate incorporation into cartilage but instead acted through a serum factor called 'sulfation factor,' later renamed 'somatomedin.' Three primary somatomedins have been identified: somatomedin C (IGF1), somatomedin A (IGF2), and somatomedin B.
Description
IGF1 A70T Human Recombinant, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 70 amino acids with an approximate molecular mass of 7.7kDa. The purification of IGF1 A70T is achieved using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
IGF1 A70T is lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB and 150 mM NaCl, at a pH of 6.0.
Solubility
To reconstitute the lyophilized IGF1 A70T, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted into other aqueous solutions as needed.
Stability
For short-term storage (up to 2-4 weeks), store the vial at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Greater than 95.0% purity as determined by SDS-PAGE.
Synonyms
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.
Source
Escherichia Coli.
Amino Acid Sequence

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKST.

Product Science Overview

What is IGF-1, Mutant A70T?

The A70T mutation refers to a specific alteration in the amino acid sequence of IGF-1. In this mutation, the amino acid alanine (A) at position 70 is replaced by threonine (T). This change can affect the protein’s structure and function, potentially altering its biological activity .

Production and Characteristics

Recombinant human IGF-1, including the A70T mutant, is produced using E. coli expression systems. The recombinant protein is typically purified and lyophilized (freeze-dried) for stability and storage . The molecular weight of IGF-1 is approximately 7.7 kDa, and it consists of a single polypeptide chain containing 70 amino acids .

Biological Activity and Applications

IGF-1 is known for its mitogenic properties, meaning it stimulates cell proliferation and survival. It has significant effects on muscle, bone, and cartilage tissue, making it a valuable tool in research and therapeutic applications . The A70T mutant of IGF-1 retains these properties but may exhibit different activity levels or stability compared to the wild-type protein.

Storage and Handling

Recombinant IGF-1, including the A70T mutant, is typically stored at -20 to -70°C to maintain its stability. It is reconstituted in sterile distilled water or an appropriate buffer before use. Proper handling and storage are crucial to preserve the protein’s biological activity .

Research and Therapeutic Potential

IGF-1 has been extensively studied for its potential in treating various conditions, including growth deficiencies, muscle wasting, and metabolic disorders. The A70T mutant, like other IGF-1 variants, is used in research to understand its specific effects and potential therapeutic applications .

In summary, Insulin-Like Growth Factor-1, Mutant A70T (Human Recombinant) is a valuable tool in scientific research, offering insights into cell growth, proliferation, and potential therapeutic applications. Its unique properties and stability make it a significant focus of study in the field of endocrinology and beyond.

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