IGF2 Human

IGF-2 Human Recombinant
Cat. No.
BT18538
Source
Escherichia Coli.
Synonyms
Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Insulin-Like Growth Factor- II Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7.5k Dalton.                  

IGF-II is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Insulin-like growth factor 2 (IGF-2) is a polypeptide growth factor belonging to the insulin gene family. This protein plays a critical role in development and growth. IGF-2 is subject to genomic imprinting, meaning its expression occurs solely from the paternally inherited allele. Research suggests its potential involvement in eating disorders. Notably, a read-through transcript, INS-IGF2, aligns with both the IGF-2 gene at its 3' end and the upstream insulin (INS) gene at its 5' end. Two alternatively spliced transcript variants of IGF-2 have been identified, both encoding the same protein.
Description
Recombinant Human Insulin-Like Growth Factor-II, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 67 amino acids. It has a molecular weight of 7.5 kDa. The purification process of IGF-II involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a sterile filtered solution containing 0.1% trifluoroacetic acid (TFA).
Solubility
To reconstitute the lyophilized IGF2, it is recommended to dissolve it in sterile 18MΩ-cm H2O at a concentration of 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized IGF-2 remains stable at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store it desiccated below -18°C. After reconstitution, IGF-II should be stored at 4°C for 2-7 days. For long-term storage, it is advisable to add a carrier protein (0.1% HSA or BSA) and store it below -18°C. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95.0% as assessed by SDS-PAGE.
Biological Activity
The biological activity is determined based on the dose-dependent proliferation of FDC-P1 cells. It is typically 8.39 ng/mL, which corresponds to a specific activity of 1.2 x 105 units/mg.
Synonyms
Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734.
Source
Escherichia Coli.
Amino Acid Sequence
AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE.

Product Science Overview

Structure and Function

IGF-2 is a single-chain polypeptide composed of 67 amino acids. It shares structural similarities with insulin and IGF-1, but its primary role is distinct. Unlike IGF-1, which has significant postnatal roles, IGF-2’s growth-promoting function is largely limited to embryonic development .

Genetic and Epigenetic Regulation

The IGF-2 gene is an imprinted gene, meaning it is expressed only from the paternal allele. This epigenetic regulation is crucial for its function. Abnormalities in the imprinting of the IGF-2 gene are associated with several disorders, including Wilms tumor, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome .

Recombinant IGF-2

Recombinant human IGF-2 is produced using E. coli expression systems. The recombinant protein is typically purified to high levels of purity, often exceeding 97%, and is used in various research and therapeutic applications . The recombinant form retains the biological activity of the native protein and is used in studies involving cell proliferation, particularly in cancer research .

Biological Activity

IGF-2 exerts its effects by binding to specific cell surface receptors, including the IGF-1 receptor and the insulin receptor. This binding activates intracellular signaling pathways that promote cell growth, differentiation, and survival. In particular, IGF-2 has been shown to stimulate cell proliferation in various cell types, including human breast cancer cells .

Clinical Implications

The role of IGF-2 in growth and development, as well as its involvement in various diseases, makes it a significant target for research. Understanding the regulation and function of IGF-2 can provide insights into developmental biology and the mechanisms underlying certain cancers and growth disorders .

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