IGF2 Mouse
Escherichia Coli.
Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734
Description
IGF2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, monomeric polypeptide chain containing 67 amino acids and having a total molecular mass of 7.4kDa.
The IGF2 is purified by proprietary chromatographic techniques.
Product Specs
Insulin-like growth factor II (IGF-II) is a crucial hormone for fetal growth, primarily produced by theca cells during pregnancy. It plays a dual role by binding to the IGF-II receptor (IGF2R), leading to its degradation, and interacting with the IGF-I receptor (IGF1R) to regulate embryonic development.
Recombinant Mouse IGF2, expressed in E. coli, is a single-chain polypeptide consisting of 67 amino acids. This non-glycosylated, monomeric protein has a molecular weight of 7.4kDa. The purification process involves proprietary chromatographic techniques.
Lyophilized from a sterile (0.2µm) filtered solution containing 0.1% trifluoroacetic acid (TFA).
For reconstitution, it is recommended to dissolve the lyophilized IGF2 in sterile water to achieve a concentration of 0.1 mg/ml. This solution can be further diluted with other aqueous solutions as needed.
Lyophilized IGF2 remains stable at room temperature for up to 3 weeks; however, for long-term storage, it is recommended to store it desiccated below -18°C. After reconstitution, the IGF2 should be stored at 4°C for 2-7 days. For extended storage, freezing below -18°C is advised, preferably with the addition of a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
The ED50, determined by FDC-P1 cell proliferation assay, is ≤ 50 ng/mL. This corresponds to a specific activity of ≥ 2.0 x 104 units/mg.
Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734
Escherichia Coli.
AYGPGETLCG GELVDTLQFV CSDRGFYFSR PSSRANRRSR GIVEECCFRS CDLALLETYC ATPAKSE
Product Science Overview
Insulin-like growth factor-2 (IGF-2) is a member of the insulin family of polypeptide growth factors, which play a crucial role in development and growth. IGF-2 is particularly significant during fetal and prenatal development, where it mediates growth-promoting activities . This protein is encoded by the IGF2 gene, which is an imprinted gene expressed predominantly from the paternal allele .
IGF-2 is a potent mitogenic growth factor that binds with high affinity to the IGF-2 receptor. It shares structural similarities with insulin but has a much higher growth-promoting activity . The IGF system, which includes IGF-1 and IGF-2, is involved in various physiological functions, including cell proliferation, differentiation, and survival .
The expression of IGF-2 is regulated by genetic and epigenetic factors. Parental imprinting plays a significant role in its regulation, with the gene being expressed only from the paternal allele . Epigenetic changes at the IGF2 locus are associated with several disorders, including Wilms tumor, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome . These conditions highlight the importance of proper IGF-2 regulation for normal growth and development.
Recombinant IGF-2, such as mouse recombinant IGF-2, is produced using recombinant DNA technology. This involves inserting the IGF2 gene into a suitable expression system, such as bacteria or mammalian cells, to produce the protein in large quantities. Recombinant IGF-2 is used in various research applications to study its role in growth and development, as well as its potential therapeutic uses .
Recombinant IGF-2 is widely used in research to understand its physiological roles and regulatory mechanisms. Studies have shown that IGF-2 is involved in tissue-specific and developmental-stage-dependent actions . It is also used to investigate the effects of IGF-2 on metabolism, tumor susceptibility, and other body functions . The availability of recombinant IGF-2 has facilitated the study of its function in various model systems, including mouse models.
